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Videos de Conceptos Relacionados

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...

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Updated: May 22, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

Las interacciones dinámicas de proteínas multicorpos sugieren vías versátiles para el tráfico de cobre.

Aaron M Keller1, Jaime J Benítez, Derek Klarin

  • 1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

Journal of the American Chemical Society
|May 15, 2012
PubMed
Resumen

El chaperón de cobre humano Hah1 le entrega el cobre a Wilson.

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Área de la Ciencia:

  • Bioquímica y Biología Molecular.
  • Mecanismos de transporte de cobre intracelular Mecanismos de transporte de cobre intracelular Mecanismos de transporte de cobre intracelular Mecanismos de transporte de cobre intracelular

Sus antecedentes:

  • El chaperón de cobre humano Hah1 facilita la entrega intracelular de cobre (Cu +) a la proteína de la enfermedad de Wilson (WDP).
  • WDP posee seis dominios de unión de metales (MBD) que reciben Cu+ de Hah1, pero el papel de múltiples MBD en el tráfico de cobre sigue sin estar claro.
  • Investigaciones anteriores exploraron las interacciones de Hah1 con MBDs WDP aislados.

Objetivo del estudio:

  • Para investigar la dinámica de interacción entre Hah1 y una construcción WDP de doble dominio (MBD34).
  • Para entender cómo Hah1 interactúa con múltiples MBDs dentro del sistema multidominio WDP.
  • Para aclarar la importancia funcional de la multiplicidad de MBD en el tráfico de cobre.

Principales métodos:

  • Empleado transferencia de energía de resonancia de fluorescencia de una sola molécula (smFRET) combinado con la captura de vesículas.
  • Probe sistemáticamente las dinámicas de interacción de Hah1-MBD3, Hah1-MBD4 y MBD3-MBD4 intramoleculares.
  • Utilizó posiciones alternas de donante y aceptador para analizar geometrías de interacción.

Principales resultados:

  • Geometrías de interacción de interconversión conservadas observadas tanto en las interacciones intermoleculares Hah1-MBD como en las intramoleculares MBD-MBD.
  • Se encontró que las interacciones Hah1-MBD dentro de la construcción MBD34 se estabilizan por un orden de magnitud en comparación con los MBD aislados.
  • La evidencia termodinámica y cinética recopilada indica que Hah1 puede interactuar simultáneamente con MBD3 y MBD4.

Conclusiones:

  • La mayor estabilidad de interacción de Hah1 con el sistema multi-MBD sugiere un mecanismo de entrega de cobre más robusto.
  • Las interacciones dinámicas intramoleculares MBD-MBD contribuyen a la versatilidad del tráfico de cobre.
  • La capacidad de Hah1 para interactuar simultáneamente con múltiples MBD de WDP apunta a una vía de transporte de cobre de Hah1 a WDP eficiente y adaptable.