Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Protein Sorting

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...

Nuclear Localization Signals and Import

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...

Nuclear Export

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...

Directionality of Nuclear Transport

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

RCC1 depletion drives protein transport defects and rupture in micronuclei.

The Journal of cell biology·2026

Same author

Long-lived cellular molecules in the brain.

Trends in neurosciences·2025

Same author

PSME3 regulates migration and differentiation of myoblasts.

Life science alliance·2025

Same author

RCC1 depletion drives protein transport defects and rupture in micronuclei.

bioRxiv : the preprint server for biology·2024

Same author

A p62-dependent rheostat dictates micronuclei catastrophe and chromosome rearrangements.

Science (New York, N.Y.)·2024

Same author

Small spaces, big problems: The abnormal nucleoplasm of micronuclei and its consequences.

Current opinion in structural biology·2024

Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026

Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026

Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026

Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026

Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026

Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026

Ver todos los artículos relacionados

Search research articles

Video Experimental Relacionado

Updated: May 7, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

La exportación de RNP por sobre nuclear en ciernes.

Emily M Hatch¹, Martin W Hetzer

¹Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

|May 15, 2012

Resumen

Este resumen es generado por máquina.

Las células musculares utilizan una nueva vía de exportación de ARNm, evitando los complejos de poros nucleares. Los complejos de ribonucleoproteínas brotan a través de la membrana nuclear para la formación de uniones neuromusculares.

Más Videos Relacionados

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Published on: June 27, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Videos de Experimentos Relacionados

Last Updated: May 7, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Published on: June 27, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología celular Biología celular.
La neurociencia es la neurociencia.

Sus antecedentes:

El complejo de poros nucleares (NPC) es la ruta establecida para la exportación de ARN mensajero (ARNm) desde el núcleo al citoplasma.
La comprensión de los mecanismos de transporte de ARNm es crucial para la regulación de la expresión génica y la función celular.

Objetivo del estudio:

Investigar mecanismos alternativos de exportación de ARNm en tipos de células especializadas.
Identificar vías para el transporte de complejos de ribonucleoproteínas a través de la envoltura nuclear.

Principales métodos:

Imágenes de células vivas del transporte de ARNm en las células musculares.
Análisis de la dinámica de la envoltura nuclear durante la formación de las uniones neuromusculares.
Caracterización bioquímica de los complejos de ribonucleoproteínas exportados.

Principales resultados:

Descubrimiento de una vía no NPC para la exportación de ARNm en células musculares.
Evidencia de complejos de ribonucleoproteínas que se fusionan con y brota a través de la membrana nuclear.
Identificación de complejos específicos involucrados en el ensamblaje de las uniones neuromusculares utilizando esta vía.

Conclusiones:

La exportación nuclear no está mediada exclusivamente por complejos de poros nucleares.
Un nuevo mecanismo de tráfico de membranas facilita la exportación de ARNm para funciones celulares específicas.
Esta vía es crítica para el desarrollo y mantenimiento de las uniones neuromusculares.