PGC7 se une a la histona H3K9me2 para proteger contra la conversión de 5mC a 5hmC en embriones tempranos
Video Experimental Relacionado
Updated: May 21, 2026
10:09
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
Published on: January 26, 2018
Toshinobu Nakamura1, Yu-Jung Liu, Hiroyuki Nakashima
1Department of Pathology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan. tnakamura@nagahama-i-bio.ac.jp
Nature
|June 23, 2012
Resumen
La proteína PGC7 mantiene la metilación del ADN impidiendo su conversión a 5-hidroximetilcitocina (5hmC). Esta regulación epigenética implica la unión a marcas histónicas específicas, cruciales para el desarrollo temprano y las células potencialmente somáticas.
Área de la Ciencia:
- La epigenética es la epigenética.
- Biología del desarrollo Biología del desarrollo.
- La genómica es la genómica.
Sus antecedentes:
- La 5-metilcitosina (5mC) es vital para la regulación epigenética de los genes durante la diferenciación celular.
- La 5-metilcitosina puede ser convertida en 5-hidroximetilcitosina (5hmC) por las enzimas Tet.
- El equilibrio de 5mC y 5hmC está relacionado con la pluripotencia y el compromiso de linaje.
Objetivo del estudio:
- Para dilucidar el mecanismo por el cual PGC7 mantiene la metilación del ADN.
- Para investigar la interacción entre las modificaciones de PGC7, Tet3 y histona.
- Para comprender el papel de PGC7 en la protección de la metilación materna y del ADN impreso.
Principales métodos:
- Los ensayos de inmunoprecipitación de cromatina (ChIP, por sus siglas en inglés) detectan la unión de PGC7.
- Análisis de los patrones de metilación e hidroximetilación del ADN.
- Estudios en modelos de ratón para evaluar la función de PGC7 in vivo.
Principales resultados:
- PGC7 se une a la cromatina materna marcada por H3K9me2.2.
- PGC7 inhibe la conversión mediada por Tet3 de 5mC a 5hmC.
- PGC7 protege los loci impresos marcados por H3K9me2 en la embriogénesis temprana.
Conclusiones:
- PGC7 protege 5mC de la conversión mediada por Tet3 al unirse a la cromatina H3K9me2.
- Este mecanismo es esencial para mantener la metilación del ADN en la embriogénesis temprana.
- Mecanismos reguladores similares pueden operar en las células somáticas.
Más Videos Relacionados
Videos de Conceptos Relacionados
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...
02:32Position-effect Variegation
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...