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Videos de Conceptos Relacionados

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

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Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron...
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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins09:55

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β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR...
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A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is...
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A Protocol for Computer-Based Protein Structure and Function Prediction16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

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Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and...
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases22:00

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Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic...
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Protein and Protein Structure

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Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
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Video Experimental Relacionado

Updated: Jan 20, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

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Predicciones de la estructura de las proteínas de membrana para la exploración.

Nick V Grishin1

  • 1Howard Hughes Medical Institute, Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9050, USA. grishin@chop.swmed.edu

Cell
|June 26, 2012
PubMed
Resumen
Este resumen es generado por máquina.

Hopf y otros. desarrolló nuevos modelos de estructura de proteínas de novo para 11 familias de proteínas transmembrana utilizando alineaciones de secuencias. Estos modelos son prometedores para la validación experimental futura y el avance de la biología estructural.

Área de la Ciencia:

  • Biología estructural Biología estructural.
  • Biología computacional Biología computacional.
  • La genómica es la genómica.

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Videos de Experimentos Relacionados

Last Updated: Jan 20, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

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A Protocol for Computer-Based Protein Structure and Function Prediction
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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Sus antecedentes:

  • Predecir las estructuras de las proteínas es crucial para comprender la función biológica.
  • Las proteínas transmembranares juegan un papel vital en los procesos celulares, pero son difíciles de estudiar estructuralmente.
  • Las alineaciones de secuencias ofrecen un recurso valioso para inferir relaciones espaciales entre residuos de aminoácidos.