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La degradación proteasomal resuelve la competencia entre la polarización celular y la cicatrización de heridas
Keiko Kono1, Yasushi Saeki, Satoshi Yoshida
1Department of Pediatric Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02115, USA.
Cell
|June 26, 2012
Resumen
La cicatrización de heridas celulares requiere redirigir los componentes celulares a los sitios dañados. Los estudios de levadura en ciernes revelan que la degradación de proteínas en los sitios de crecimiento polarizados es clave para evitar la competencia durante la reparación de la membrana.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La cicatrización de heridas celulares es vital para los eucariotas, ya que implica la redirección de la maquinaria citoesquelética y secretora a los sitios dañados.
- Se identifican proteínas conservadas en la cicatrización de heridas, pero los mecanismos que las vinculan siguen sin estar claros.
Objetivo del estudio:
- Investigar los mecanismos que unen los componentes celulares durante la cicatrización de heridas en células polarizadas.
- Para comprender cómo la levadura en ciernes (Saccharomyces cerevisiae) responde al daño de la pared celular / membrana inducido por láser.
Principales métodos:
- Utilizado el daño inducido por láser en la levadura en ciernes para estudiar la cicatrización de heridas celulares.
- Investigó la dispersión de proteínas desde sitios de crecimiento polarizados hasta sitios de heridas.
- Se han definido las vías de degradación dependientes de la proteína quinasa C (PKC).
Principales resultados:
- El daño local de la pared celular / membrana desencadena la dispersión de proteínas desde los sitios de crecimiento polarizados hasta la herida.
- Un mecanismo dependiente de la proteína quinasa C media la destrucción de la formina Bni1 y el componente del exocisto Sec3.
- Esta degradación es crucial para evitar la competencia entre el crecimiento polarizado y los sitios de cicatrización de heridas.
Conclusiones:
- La dispersión de proteínas de los sitios de crecimiento polarizados es una estrategia clave de curación de heridas en la levadura en ciernes.
- Los mecanismos de degradación de las proteínas son esenciales para resolver la competencia entre distintos procesos celulares durante la reparación.
- Superar la competencia de los citoesqueletos polarizados preexistentes puede ser una característica general de la cicatrización de heridas en las células polarizadas.
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