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La señalización de la muesca miocárdica reprograma los cardiomiocitos a un fenotipo similar a la conducción
Stacey Rentschler1, Alberta H Yen, Jia Lu
1Cardiovascular Institute, University of Pennsylvania, 421 Curie Blvd, Philadelphia, PA 19104, USA. srentsch@dom.wustl.edu
Circulation
|July 28, 2012
Resumen
La señalización de la muesca guía el desarrollo de las células cardíacas, instruyendo a los progenitores cardíacos para que se conviertan en células de conducción especializadas. Este descubrimiento es prometedor para la ingeniería de tejidos cardiovasculares.
Área de la Ciencia:
- Biología Cardiovascular Biología Cardiovascular
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
Sus antecedentes:
- La señalización de muescas regula el destino celular y la diferenciación en la cardiogénesis entre especies.
- Su papel en la restricción progresiva del linaje de los cardiomiocitos en las células de conducción es inexplorado.
Objetivo del estudio:
- Para investigar el papel de la señalización de Notch en la especialización de los cardiomiocitos.
- Para determinar si Notch puede dirigir los progenitores cardíacos hacia un destino celular de conducción.
Principales métodos:
- Activación de la señalización de Notch en el miocardio durante el desarrollo temprano.
- Activación in vitro de Notch en cardiomiocitos de recién nacidos.
- Análisis del transcriptoma y evaluaciones electrofisiológicas.
Principales resultados:
- La señalización de Notch activada promueve un fenotipo similar a la conducción en el corazón.
- Se observó la expresión ectópica de genes específicos del sistema de conducción.
- Los estudios in vitro mostraron cambios globales en el transcriptoma y características alteradas del potencial de acción, lo que indica una reprogramación.
Conclusiones:
- La señalización de muesca puede instruir a los progenitores cardíacos de cámara para que se diferencien en células similares a la conducción.
- Los cardiomiocitos en etapa tardía conservan la plasticidad, lo que sugiere un potencial para la ingeniería de tejidos cardiovasculares.
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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