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Definir el núcleo del microbioma de la raíz de Arabidopsis thaliana
Derek S Lundberg1,2, Sarah L Lebeis1, Sur Herrera Paredes1
1Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Nature
|August 4, 2012
Resumen
Las raíces de las plantas albergan distintas comunidades microbianas, y el tipo de suelo influye significativamente en la composición de la microbiota de las raíces. Grupos bacterianos específicos dominan el compartimiento endofítico de baja complejidad, ofreciendo información sobre las interacciones entre plantas y microbios.
Área de la Ciencia:
- Biología vegetal Biología vegetal
- Microbiología Microbiología.
- Genética La genética.
Sus antecedentes:
- Las plantas terrestres albergan comunidades microbianas asociadas a las raíces distintas del suelo a granel.
- La rizosfera y la microbiota endofítica impactan el crecimiento de las plantas, la productividad y los servicios del ecosistema.
- Comprender cómo las plantas anfitrionas seleccionan microbios específicos del suelo es crucial pero poco entendido.
Objetivo del estudio:
- Para investigar la especificidad del huésped de la microbiota de la raíz en Arabidopsis thaliana.
- Para determinar si las comunidades microbianas de las raíces son consistentes a través de los tipos de suelo y las etapas de desarrollo de las plantas.
- Evaluar la influencia del genotipo del huésped en las comunidades bacterianas asociadas a las raíces.
Principales métodos:
- Pirosecuenciación del gen del ARN ribosomal 16S bacteriano.
- Análisis de más de 600 plantas de Arabidopsis thaliana cultivadas en dos suelos distintos.
- Caracterización de las comunidades microbianas en el suelo a granel, la rizosfera y los compartimentos endofíticos.
Principales resultados:
- Las comunidades bacterianas diferían significativamente entre los suelos a granel, la rizosfera y los compartimentos endofíticos.
- El tipo de suelo influyó fuertemente en la composición de la comunidad microbiana en todos los compartimentos.
- Los compartimentos endofíticos exhibieron comunidades de baja complejidad enriquecidas con Actinobacteria y Proteobacteria.
- Algunos taxones bacterianos variaron cuantitativamente con la etapa de desarrollo de la planta y el genotipo.
Conclusiones:
- La composición de la microbiota de las raíces está significativamente determinada por el tipo de suelo.
- Las comunidades endofíticas son distintas y menos complejas que las comunidades de la rizosfera.
- El genotipo del huésped y la etapa de desarrollo tienen una influencia cuantitativa en bacterias específicas asociadas a la raíz.
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