Video Experimental Relacionado
Updated: May 18, 2026
08:57
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
BATF-JUN es crítico para la transcripción mediada por IRF4 en las células T
Peng Li1, Rosanne Spolski, Wei Liao
1Laboratory of Molecular Immunology and Immunology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1674, USA. lip3@nhlbi.nih.gov
Nature
|September 21, 2012
Resumen
El factor regulador del interferón 4 (IRF4) coopera con los complejos AP1 en las células T, no solo con PU.1 en las células B. Esta interacción IRF4-AP1 regula genes como el Il10, ofreciendo nuevas dianas terapéuticas.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- El Reglamento del Factor de Transcripción.
Sus antecedentes:
- El factor regulador del interferón 4 (IRF4) es crucial para el desarrollo linfoide y las respuestas inmunes.
- La unión al ADN de IRF4 es típicamente débil, mejorada por factores como PU.1 en las células B para regular los elementos compuestos ETS-IRF (EICE).
Objetivo del estudio:
- Investigar nuevos mecanismos de unión al ADN de IRF4 y la regulación de la transcripción en las células T CD4 (((+).
- Para identificar socios cooperativos de IRF4 más allá de PU.1/SPIB en diferentes tipos de células inmunes.
Principales métodos:
- Análisis de la unión de IRF4 a motivos compuestos de ADN en células T y células B CD4 ((+) de ratón.
- Se ha investigado la unión cooperativa con la proteína activadora 1 (AP1) y las proteínas de la familia BATF-JUN.
- Evaluar la regulación de la transcripción génica, incluido el gen Il10, en células T de tipo salvaje y knockout.
Principales resultados:
- IRF4 coopera inesperadamente con los complejos AP1 para unirse a los elementos compuestos AP1-IRF (AICE) en las células T CD4 ((+)).
- Las proteínas BATF-JUN cooperan con IRF4 en AICEs en células T activadas y T ((H) 17 diferenciadas CD4 ((+) T.
- La unión cooperativa de IRF4 y AP1/BATF mejora la transcripción del gen Il10 regulado por IL-21.
Conclusiones:
- IRF4 utiliza distintos socios de unión al ADN (ETS o AP1) dependiendo del contexto celular.
- Esto revela un nuevo modo de regulación génica mediada por IRF4 a través de la cooperación AP1 en las células T.
- Los hallazgos sugieren nuevas estrategias para modular las respuestas inmunes dependientes de la IRF4.
Videos de Conceptos Relacionados
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
02:26NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...