Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

The application of Global Burden of Animal Diseases methodology to aquatic animal production.

Revue scientifique et technique (International Office of Epizootics)·2024
Same author

Interpretation and utility of the Animal Health Loss Envelope as part of the Global Burden of Animal Diseases analytical process.

Revue scientifique et technique (International Office of Epizootics)·2024
Same author

Farm level bio-economic modelling of aquatic animal disease and health interventions.

Preventive veterinary medicine·2023
Same author

Global Burden of Animal Diseases: a novel approach to understanding and managing disease in livestock and aquaculture.

Revue scientifique et technique (International Office of Epizootics)·2021
Same author

Coherent spin qubit transport in silicon.

Nature communications·2021
Same author

Review: Mitigating the risks posed by intensification in livestock production: the examples of antimicrobial resistance and zoonoses.

Animal : an international journal of animal bioscience·2021

Video Experimental Relacionado

Updated: May 11, 2026

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 15, 2014

Las mutaciones pleiotrópicas dentro de dos genes mitocondriales del citocromo de la levadura bloquean el

G M Church, P P Slonimski, W Gilbert

    Cell
    |December 1, 1979
    PubMed
    Resumen

    Las mutaciones genéticas mitocondriales de la levadura afectan el procesamiento del ARN. Algunas mutaciones en el citocromo b interrumpen un ARN requerido, mientras que las mutaciones oxi-3 pueden crear una actividad inhibidora que afecta la maduración del ARN.

    Más Videos Relacionados

    Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
    08:33

    Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

    Published on: April 11, 2021

    Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly
    09:53

    Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly

    Published on: June 7, 2024

    Videos de Experimentos Relacionados

    Last Updated: May 11, 2026

    Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
    14:44

    Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

    Published on: March 15, 2014

    Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
    08:33

    Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

    Published on: April 11, 2021

    Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly
    09:53

    Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly

    Published on: June 7, 2024

    Área de la Ciencia:

    • Genética mitocondrial genética mitocondrial
    • Procesamiento de ARN y procesamiento de ARN.
    • Biología molecular La biología molecular.

    Sus antecedentes:

    • Los genes mitocondriales de la levadura, incluidos el cob-box (citocromo b) y el oxi-3 (subunidad de citocromo oxidasa 40,000 dalton), codifican proteínas esenciales.
    • Estos genes se transcriben en grandes precursores de ARNm (7-10 kb) que se someten a un procesamiento complejo.
    • El procesamiento de ARN es crucial para generar ARN mensajeros funcionales (ARNm) para la síntesis de proteínas.

    Objetivo del estudio:

    • Para investigar el papel de mutaciones específicas en los genes mitocondriales de la levadura en el procesamiento del ARNm.
    • Identificar los mecanismos moleculares por los cuales las mutaciones en los genes cob-box y oxi-3 afectan la maduración del ARN.
    • Para entender la interacción entre los diferentes genes mitocondriales en la vía de procesamiento.

    Principales métodos:

    • Análisis de los pasos de procesamiento del ARN en levaduras mutantes.
    • Caracterización de las mutaciones que afectan a la expresión génica de cob-box y oxi-3.
    • Investigando la naturaleza de los factores de acción trans involucrados en la maduración del ARNm.

    Principales resultados:

    • Las mutaciones en el gen cob-box conducen a efectos pleiotrópicos, bloqueando la maduración del ARN en varios pasos.
    • Estos mutantes del citocromo b parecen carecer de un ARN trans-activo funcional esencial para el procesamiento de los ARNm cob-box y oxi-3.
    • Las mutaciones en el gen oxi-3 pueden dar lugar a la producción de una actividad inhibidora que interfiere con pasos específicos de procesamiento de ARN.

    Conclusiones:

    • El procesamiento de los ARNm cob-box y oxi-3 es interdependiente y está regulado por factores específicos.
    • Un ARN de acción trans es crítico para la maduración coordinada de estos ARNm mitocondriales.
    • Las mutaciones Oxi-3 pueden interrumpir el procesamiento de ARN a través de mecanismos inhibidores, destacando vías reguladoras complejas.