Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Prevalence and predictors of coeliac disease in patients with irritable bowel syndrome: A prospective study from Rajasthan, India.

Tropical doctor·2026
Same author

A tunable Cas12a platform for single-cell perturbation screening and CRISPRi.

Nature communications·2026
Same author

Human haematopoietic stem cells remember inflammatory stress.

Nature·2026
Same author

Visceral Muscle Dysmotility Syndrome With Esophageal Aperistalsis (VMDS Tetrad): A Rare Initial Manifestation of Systemic Lupus Erythematosus.

ACG case reports journal·2026
Same author

Near infrared fluorescence-guided lymphadenectomy during esophagectomy for esophageal squamous cell carcinoma.

Surgery·2026
Same author

Invasive mould infections of the central nervous system in the Indian population: a cohort study (2004-2025).

The Lancet regional health. Southeast Asia·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
Same journal

Structural basis for host membrane binding and remodeling by invading malaria parasites.

Cell·2026
Same journal

Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning.

Cell·2026
Same journal

Arc mediates intercellular tau transmission via extracellular vesicles.

Cell·2026
Same journal

Geometric constraints on the architecture of mammalian cortical connectomes.

Cell·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: May 10, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Una red reguladora validada para la especificación de células Th17.

Maria Ciofani1, Aviv Madar, Carolina Galan

  • 1Molecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.

Cell
|October 2, 2012
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores mapearon la red reguladora que controla la diferenciación celular del T ayudante 17 (Th17). Esto revela factores clave de transcripción como RORγt y BATF, ofreciendo nuevos objetivos terapéuticos para enfermedades inflamatorias.

Más Videos Relacionados

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

Videos de Experimentos Relacionados

Last Updated: May 10, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

Área de la Ciencia:

  • Inmunología Inmunología.
  • Biología Molecular Biología Molecular
  • Biología de Sistemas Biología de Sistemas.

Sus antecedentes:

  • Las células T ayudantes 17 (Th17) son cruciales para la inmunidad de la mucosa, pero también están implicadas en enfermedades inflamatorias.
  • La diferenciación celular Th17 está orquestada por una compleja interacción de factores de transcripción (TF), incluido RORγt.

Objetivo del estudio:

  • Delinear la red reguladora transcripcional global que rige la diferenciación de las células Th17.
  • Identificar nuevos reguladores y comprender las funciones jerárquicas de los TF clave.

Principales métodos:

  • Utilizó un enfoque iterativo de biología de sistemas que combina ocupación de TF en todo el genoma, perfiles de expresión de mutantes TF y datos de expresión de series temporales.
  • Integró múltiples conjuntos de datos para construir y validar un modelo predictivo de la red reguladora Th17.

Principales resultados:

  • Se identificaron los BATF e IRF4 como críticos para la accesibilidad inicial de la cromatina y el inicio de la transcripción.
  • Se demostró que RORγt juega un papel focal y determinista en la sintonización global del programa transcripcional Th17.
  • Descubrió nuevos reguladores de Th17, incluido Fosl2, destacando su papel en la plasticidad celular.

Conclusiones:

  • El estudio presenta un mapa completo de la red reguladora transcripcional Th17.
  • El modelo predictivo validado ofrece información sobre la cooperación TF y el control jerárquico.
  • Los reguladores identificados y la dinámica de la red proporcionan una base para el desarrollo de nuevas estrategias terapéuticas para las enfermedades inflamatorias mediadas por Th17.