Video Experimental Relacionado
Updated: May 17, 2026
09:10
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
Published on: October 28, 2019
Regulación mediada por akt de la autofagia y la tumorigénesis a través de la fosforilación de Beclin 1
Richard C Wang1, Yongjie Wei, Zhenyi An
1Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Science (New York, N.Y.)
|November 1, 2012
Resumen
La señalización aberrante a través de la vía de la fosfatidilinositol 3-cinasa (PI3K) -Akt promueve el cáncer. Akt fosforila Beclin 1, inhibiendo la autofagia y promoviendo el crecimiento tumoral mediante la formación de un complejo Beclin 1/14-3-3/vimentin.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- La señalización aberrante a través de la vía phosphatidylinositol 3-kinase (PI3K) -Akt de clase I es un sello distintivo de los cánceres humanos.
- Beclin 1 es una proteína crucial involucrada en la autofagia y actúa como supresor de tumores.
Objetivo del estudio:
- Investigar el papel de la vía de señalización Akt en la regulación de la función de Beclin 1.
- Aclarar el mecanismo por el cual Akt influye en la autofagia y la tumorigénesis a través de Beclin 1.
Principales métodos:
- Utilizó un mutante Beclin 1 resistente a la fosforilación mediada por Akt.
- Se evaluaron los niveles de autofagia, el crecimiento independiente del anclaje y la tumorigénesis en respuesta al mutante Beclin 1.
- Se examinaron las interacciones proteína-proteína entre Beclin 1, 14-3-3 y vimentin.
- Investigó el efecto del agotamiento de la vimentina en la autofagia y la transformación.
Principales resultados:
- La expresión de un mutante Beclin 1 resistente a Akt aumentó la autofagia, redujo el crecimiento independiente del anclaje e inhibió la tumorigénesis impulsada por Akt.
- La fosforilación mediada por akt de Beclin 1 aumentó su interacción con 14-3-3 y vimentin.
- El agotamiento de la vimentina condujo a un aumento de la autofagia e inhibió la transformación impulsada por Akt.
- Se identificó un complejo inhibidor de la autofagia que involucra a Beclin 1, 14-3-3 y vimentin.
Conclusiones:
- La fosforilación mediada por Akt de Beclin 1 es un mecanismo clave para inhibir la autofagia y promover la oncogénesis.
- El complejo Beclin 1/14-3-3/vimentin juega un papel importante en la progresión del cáncer impulsada por Akt.
- Estos hallazgos resaltan la interacción entre la señalización Akt, los filamentos intermedios y la autofagia en el cáncer, ofreciendo objetivos terapéuticos potenciales.
Videos de Conceptos Relacionados
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
01:27Autophagy
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
01:18Autophagic Cell Death
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
01:36Delivery Pathways to the Lysosome
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...