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Una enzima metabólica central media la resistencia al gas fosfina
David I Schlipalius1, Nicholas Valmas, Andrew G Tuck
1Agri-Science Queensland, Department of Agriculture, Fisheries and Forestry, Ecosciences Precinct, Brisbane, QLD 4001, Australia.
Science (New York, N.Y.)
|November 10, 2012
Resumen
La resistencia genética al gas fosfina, un protector de granos, está relacionada con mutaciones en la enzima dihidrolipoamida deshidrogenasa (DLD). Este descubrimiento puede conducir a nuevas estrategias para controlar plagas resistentes y proteger la seguridad alimentaria.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Manejo de plagas de gestión de plagas.
Sus antecedentes:
- La fosfina es un fumigante crucial para proteger las reservas mundiales de granos.
- La emergente resistencia a la fosfina en insectos plaga plantea una amenaza significativa para la seguridad alimentaria.
- La dihidrolipoamida deshidrogenasa (DLD) es una enzima metabólica clave.
Objetivo del estudio:
- Investigar las bases genéticas de la resistencia a la fosfina en insectos plagas y nematodos.
- Identificar nuevos mecanismos de resistencia y objetivos potenciales para las estrategias de control.
Principales métodos:
- Análisis de los polimorfismos genéticos en los insectos resistentes a la fosfina (Rhyzopertha dominica, Tribolium castaneum) y los nematodos (Caenorhabditis elegans).
- Investigando el papel de la dihidrolipoamida deshidrogenasa (DLD) en la resistencia a la fosfina.
- Perfiles metabólicos para evaluar los cambios metabólicos tras la exposición a la fosfina.
Principales resultados:
- La resistencia genética a la fosfina está asociada con polimorfismos en la dihidrolipoamida deshidrogenasa (DLD).
- Estas mutaciones se localizan cerca de la interfaz catalítica de disulfuro o dimerización de la enzima.
- Las mutaciones DLD alteran las vías metabólicas centrales y aumentan la sensibilidad a la arsenita en C. elegans.
Conclusiones:
- La dihidrolipoamida deshidrogenasa (DLD) representa una nueva clase de factor de resistencia para las toxinas redox activas.
- Aprovechar el aumento de la sensibilidad al arsenito en los mutantes de DLD podría proporcionar un nuevo enfoque para controlar las plagas resistentes a la fosfina.
- Esta investigación ofrece soluciones potenciales para salvaguardar la seguridad alimentaria mundial contra los insectos resistentes.
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