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La estructura de las ribonucleoproteínas del virión de la gripe nativa

Rocío Arranz¹, Rocío Coloma, Francisco Javier Chichón

¹Department of Macromolecular Structure, Centro Nacional de Biotecnología [Consejo Superior de Investigaciones Cienfícas (CSIC)], Madrid, Spain.

Science (New York, N.Y.)

|November 28, 2012

Resumen

Las ribonucleoproteínas del virus de la gripe (RNP) tienen una estructura de doble hélice. Este hallazgo revela cómo se organizan los RNP de la influenza dentro del virus, lo que afecta la transcripción, la replicación y el ensamblaje.

Área de la Ciencia:

Virología Virología.
Biología Estructural Biología estructural.
Biología Molecular Biología Molecular

Sus antecedentes:

Los virus de la gripe causan importantes problemas mundiales de salud pública a través de epidemias y pandemias anuales.
La transcripción y la replicación del virus dependen de las ribonucleoproteínas (RNP), complejos de ARN segmentados de cadena negativa, nucleoproteína (NP) y el complejo polimerasa.

Objetivo del estudio:

Para dilucidar la organización estructural de las ribonucleoproteínas nativas del virus de la gripe (RNP) derivadas directamente de los viriones.

Principales métodos:

Análisis estructural de los RNP nativos aislados de los virus de la influenza.

Principales resultados:

Las RNP de influenza nativa adoptan una conformación de doble hélice.
Dos hebras de nucleoproteína (NP) de polaridad opuesta se asocian a lo largo de la hélice.
Las hebras están unidas por un bucle en un extremo e interactúan con el complejo polimerasa en el otro.

Conclusiones:

La estructura RNP descrita proporciona información sobre los mecanismos de transcripción y replicación del ARN viral.
La comprensión de la estructura de RNP es crucial para investigar los procesos de importación y encapsulación nuclear.
Esta información estructural puede informar estrategias para combatir la propagación del virus de la influenza.

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