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Descifrar y predecir la dinámica del transcriptoma bajo condiciones de campo fluctuantes
Atsushi J Nagano1, Yutaka Sato, Motohiro Mihara
1Photosynthesis and Photobiology Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Cell
|December 11, 2012
Resumen
Las plantas de arroz las plantas de arroz.
Área de la Ciencia:
- Biología Vegetal Biología Vegetal
- La genómica es la genómica.
- Ciencias del medio ambiente Ciencias del medio ambiente.
Sus antecedentes:
- La expresión génica es compleja en entornos naturales en comparación con los laboratorios.
- Comprender las influencias ambientales en la expresión génica de las plantas es crucial para la agricultura.
Objetivo del estudio:
- Para modelar los factores endógenos y externos que afectan la expresión génica de la planta de arroz en un campo de arroz.
- Para identificar los principales impulsores de la dinámica del transcriptoma en un entorno natural.
Principales métodos:
- Recogió datos de transcriptomas de hojas de arroz.
- Recogió los datos meteorológicos correspondientes.
- Desarrolló modelos estadísticos para analizar los impulsores de la expresión génica.
Principales resultados:
- La dinámica del transcriptoma está impulsada principalmente por los ritmos diurnos, la temperatura, la edad de la planta y la radiación solar.
- Tiempos específicos identificados (puertas diurnas) cuando los factores ambientales influyen en la transcripción génica.
- Cuantificó el impacto relativo de los factores circadianos y ambientales en los genes metabólicos.
Conclusiones:
- Desarrolló modelos predictivos para los efectos de la temperatura en la dinámica del transcriptoma del arroz.
- El estudio proporciona ideas aplicables a los desafíos agrícolas.
- El enfoque puede extenderse para descifrar las fluctuaciones del transcriptoma en otros organismos.
Videos de Conceptos Relacionados
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

