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La arquitectura del complejo del núcleo del factor de transcripción general humano TFIID
Christoph Bieniossek1, Gabor Papai, Christiane Schaffitzel
1European Molecular Biology Laboratory Grenoble Outstation, Unit of Virus Host Cell Interactions UVHCI, UJF-CNRS-EMBL Unité Mixte International UMI 3265, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
Nature
|January 8, 2013
Resumen
La estructura del núcleo humano-TFIID revela una arquitectura simétrica que transiciona a la asimetría en la unión TAF8-TAF10, guiando el ensamblaje holo-TFIID para la regulación de la transcripción génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología Estructural Biología estructural.
- Reglamento genético Reglamento genético.
Sus antecedentes:
- El factor de transcripción IID (TFIID) es esencial para la iniciación de la transcripción génica por la ARN polimerasa II.
- TFIID es un gran complejo de proteína de unión a la caja TATA (TBP) y 13 factores asociados a TBP (TAF).
- La arquitectura precisa y la vía de ensamblaje de TFIID siguen siendo en gran medida desconocidas.
Objetivo del estudio:
- Para determinar la estructura de alta resolución del núcleo humano-TFIID.
- Para aclarar el papel de la simetría núcleo-TFIID en el ensamblaje de holo-TFIID.
- Para entender cómo el TFIID facilita la transcripción génica.
Principales métodos:
- Microscopía cryoelectrónica (cryo-EM) con una resolución de 11,6 Å.
- Análisis estructural del núcleo humano-TFIID.
- Ensayos bioquímicos para estudiar la unión al complejo TAF8-TAF10.
Principales resultados:
- La estructura del núcleo humano -TFIID- exhibe una doble arquitectura simétrica y entrelazada.
- Las características estructurales conservadas de los TAF, incluidos los pliegues de histonas, se acomodan dentro de la estructura del núcleo-TFIID.
- La unión del complejo TAF8-TAF10 interrumpe la simetría del núcleo-TFIID, creando un andamio asimétrico.
Conclusiones:
- La estructura asimétrica del núcleo-TFIID sirve como un sitio de nucleación para el ensamblaje del holo-TFIID.
- Esta transición estructural de la simetría a la asimetría es crucial para la vía de ensamblaje TFIID.
- Comprender la estructura de TFIID proporciona información sobre la regulación de la transcripción génica.
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