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El virión del bacteriófago t7 sufre una extensa remodelación estructural durante la infección
Bo Hu1, William Margolin, Ian J Molineux
1Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, TX 77030, USA.
Science (New York, N.Y.)
|January 12, 2013
Resumen
La infección por el bacteriófago T7 implica que las fibras de la cola se extienden después de la adsorción, formando un conducto para la eyección del ADN. La cola luego se derrumba, permitiendo que la membrana celular huésped se vuelva a sellar.
Área de la Ciencia:
- Microbiología Microbiología.
- Biología Estructural Biología estructural.
- Virología Virología.
Sus antecedentes:
- La adsorción de los bacteriófagos y la eyección del genoma son fundamentales para su ciclo de vida.
- Los mecanismos moleculares precisos que subyacen a estos procesos siguen siendo incompletamente entendidos.
- El bacteriófago T7 es un organismo modelo para el estudio de los mecanismos de infección viral.
Objetivo del estudio:
- Para dilucidar los mecanismos estructurales de la adsorción del bacteriófago T7 y la eyección del genoma.
- Para visualizar los cambios estructurales dinámicos de los viriones T7 durante la infección de la célula huésped.
- Para proporcionar una visión estructural de alta resolución en el proceso de eyección de ADN.
Principales métodos:
- Se empleó tomografía criolectrónica (Cryo-ET) para capturar viriones T7.
- Las etapas de infección se estudiaron utilizando minicélulas de Escherichia coli.
- El análisis estructural se realizó a una resolución de aproximadamente 4 nm.
Principales resultados:
- Las fibras de la cola de los fagos se extienden y se orientan simétricamente solo después de una adsorción exitosa.
- La unión al receptor indujo la extensión de la cola, formando un conducto de eyección de ADN.
- Después de la eyección, la estructura de la cola se alteró, lo que facilita el nuevo sellado de la membrana celular del huésped.
Conclusiones:
- El estudio proporciona una serie estructural detallada de los intermediarios durante la infección del fago T7.
- Cryo-ET revela los cambios dinámicos de conformación de la cola del bacteriófago durante la infección.
- Estos hallazgos mejoran nuestra comprensión de las interacciones entre bacteriófagos y huéspedes y la entrega de ADN.
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