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DBP, un activador transcripcional enriquecido con hígado, se expresa tarde en la ontogenia y su especificidad tisular
C R Mueller1, P Maire, U Schibler
1Department of Molecular Biology, University of Geneva, Switzerland.
Cell
|April 20, 1990
Resumen
El activador transcripcional DBP, una proteína involucrada en la regulación del gen de la albúmina, está altamente expresado en el hígado adulto. Su expresión está regulada a la baja durante la regeneración hepática, lo que sugiere un papel en el control de la proliferación de los hepatocitos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación genética Reglamento genético.
- Función de los hepatocitos.
Sus antecedentes:
- El promotor de albúmina contiene un sitio D reconocido por un activador transcripcional.
- Los factores de transcripción son reguladores cruciales de la expresión génica.
- La familia de proteínas DBP comparte un dominio básico pero DBP carece de una cremallera de leucina.
Objetivo del estudio:
- Clonar y caracterizar el activador de la transcripción DBP.
- Para investigar la distribución tisular y la expresión del desarrollo de DBP.
- Explorar el papel de la DBP en la regeneración hepática y la proliferación celular.
Principales métodos:
- La clonación completa de ADNc de DBP.
- Análisis de los niveles de proteína DBP y ARNm en varios tejidos de ratas.
- Examen de la expresión de DBP durante la regeneración hepática inducida químicamente.
Principales resultados:
- El ADNc de DBP fue clonado, revelando un factor de transcripción relacionado con Fos, Jun, CREB y C/EBP.
- La proteína DBP se encuentra predominantemente en el hígado de la rata adulta, mientras que su ARNm está muy extendido pero se acumula en los adultos.
- La expresión de DBP se desregula rápidamente durante la regeneración hepática, y esta regulación se produce a nivel de ARNm.
Conclusiones:
- DBP es un nuevo activador transcripcional que se une al sitio D del promotor de la albúmina.
- DBP exhibe regulación específica del tejido y del desarrollo, principalmente en el hígado adulto.
- La regulación a la baja de la DBP durante la regeneración hepática sugiere un papel en el control de la proliferación de hepatocitos.
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