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Ex vivo Culturing of Whole, Developing Drosophila Brains
Published on: July 27, 2012
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La reorganización del genoma subnuclear regulada por el desarrollo restringe la competencia del progenitor neural en
Minoree Kohwi1, Joshua R Lupton, Sen-Lin Lai
1Institute of Neuroscience, Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA.
Cell
|January 22, 2013
Resumen
Las células madre pierden su capacidad para crear tipos celulares tempranos con el tiempo. En Drosophila, el gen de jorobado.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- Las células madre y las células progenitoras generan tipos celulares distintos secuencialmente.
- Los progenitores pierden la capacidad de especificar destinos tempranos con el tiempo a través de mecanismos desconocidos.
- En Drosophila, el factor de transcripción del jorobado (Hb) especifica las neuronas tempranas en los progenitores neurales (neuroblastos).
Objetivo del estudio:
- Investigar los mecanismos subyacentes a la pérdida de la competencia progenitor en Drosophila.
- Comprender el papel de la regulación genética del jorobado en la especificación del destino de los neuroblastos.
Principales métodos:
- In vivo inmuno-ADN FISH para rastrear la ubicación de los genes.
- Análisis del reposicionamiento del gen del jorobado dentro del núcleo.
- Correlación del reposicionamiento génico con la expresión de proteínas (antena distal - Dan) y la competencia del progenitor.
Principales resultados:
- El gen del Jorobado se mueve a la periferia nuclear, un compartimento represivo, a medida que se pierde la competencia de los neuroblastos.
- Este reposicionamiento se produce horas después de la terminación de la transcripción y se correlaciona con la regulación a la baja de la antena distal (Dan).
- Prolongar la expresión de Dan o interrumpir la lámina impidió el reposicionamiento del jorobado y amplió la competencia de los neuroblastos.
Conclusiones:
- Los neuroblastos se someten a una reorganización del genoma subnuclear regulada por el desarrollo.
- Esta reorganización silencia permanentemente los genes diana del jorobado, lo que lleva a la pérdida de la competencia del progenitor.
- El posicionamiento de genes subnucleares es un mecanismo clave que regula el tiempo de desarrollo y las decisiones del destino celular.
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