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Videos de Conceptos Relacionados

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Video Experimental Relacionado

Updated: May 14, 2026

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

Braveheart, un largo ARN no codificante requerido para el compromiso del linaje cardiovascular.

Carla A Klattenhoff1, Johanna C Scheuermann, Lauren E Surface

  • 1Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Cell
|January 29, 2013
PubMed
Resumen

Braveheart (Bvht), un nuevo ARN largo no codificante, es crucial para establecer el linaje cardiovascular. Este lncRNA asociado al corazón guía la diferenciación de las células madre embrionarias hacia el destino cardíaco y regula los genes clave del desarrollo.

Videos de Experimentos Relacionados

Last Updated: May 14, 2026

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

Área de la Ciencia:

  • Biología del desarrollo Biología del desarrollo.
  • La epigenética es la epigenética.
  • ARN Biología Biología ARN

Sus antecedentes:

  • Los ARN largos no codificantes (ARNlnc) juegan un papel en el desarrollo, pero su función en el compromiso con el linaje es en gran medida desconocida.
  • Comprender los mecanismos moleculares del desarrollo cardiovascular es fundamental para la medicina regenerativa.

Objetivo del estudio:

  • Identificar y caracterizar nuevos lncRNAs involucrados en el compromiso del linaje cardiovascular.
  • Para aclarar la función del lncRNA Braveheart (Bvht) asociado al corazón en el desarrollo del corazón de los mamíferos.

Principales métodos:

  • Ensayos de diferenciación de células madre embrionarias (CEM).
  • Análisis de la expresión génica (qRT-PCR, RNA-seq).
  • Los ensayos de inmunoprecipitación de cromatina (ChIP, por sus siglas en inglés) evalúan las modificaciones epigenéticas.
  • Estudios de interacción con SUZ12 y MesP1.1.

Principales resultados:

  • Bvht es esencial para la diferenciación del mesodermo en linajes cardíacos.
  • Bvht regula una red genética cardiovascular central y actúa aguas arriba de MesP1.1.
  • Bvht interactúa con SUZ12, un componente de PRC2, lo que sugiere un papel en la regulación epigenética.
  • Bvht también está involucrado en el mantenimiento del destino cardíaco en los cardiomiocitos neonatales.

Conclusiones:

  • Braveheart (Bvht) es un ARN no codificante largo crítico para el establecimiento del linaje cardiovascular durante el desarrollo de los mamíferos.
  • Funciona a través de mecanismos epigenéticos que involucran a PRC2 para regular el compromiso cardíaco.
  • Este estudio pone de relieve la importancia de los lncRNA en los procesos de desarrollo y proporciona un nuevo objetivo para la investigación cardíaca.