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Videos de Conceptos Relacionados

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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Video Experimental Relacionado

Updated: May 14, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Los ensamblajes dinámicos de ADN mediados por el desplazamiento de la cadena de ADN inducido por la unión.

Feng Li1, Hongquan Zhang, Zhixin Wang

  • 1Department of Chemistry, University of Alberta, Edmonton, Canada T6G 2G3.

Journal of the American Chemical Society
|January 31, 2013
PubMed
Resumen

Los investigadores desarrollaron una nueva estrategia de desplazamiento de cadenas de ADN. Este método permite que la unión a proteínas desencadene ensamblajes dinámicos de ADN, expandiendo su uso en diagnósticos y terapias.

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Last Updated: May 14, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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Design and Synthesis of a Reconfigurable DNA Accordion Rack

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Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Molecular Biología Molecular
  • Nanotecnología La nanotecnología es la nanotecnología.

Sus antecedentes:

  • Los ensamblajes dinámicos de ADN ofrecen potencial en la regulación celular, la terapéutica y el diagnóstico.
  • Las limitaciones actuales restringen las aplicaciones de ensamblaje de ADN a los ácidos nucleicos y las moléculas pequeñas debido a los mecanismos de activación limitados.

Objetivo del estudio:

  • Introducir una nueva estrategia de desplazamiento de cadenas de ADN para ensamblajes dinámicos de ADN sensibles a las proteínas.
  • Para superar las limitaciones de los métodos de activación de ensamblaje de ADN existentes.

Principales métodos:

  • Desarrolló una estrategia de desplazamiento de cadena de ADN inducida por unión.
  • Conversión demostrada de eventos de unión a proteínas en liberación de salida de ADN prediseñado.
  • Hacía funcionar el sistema a temperatura ambiente.

Principales resultados:

  • Se logra una alta eficiencia de conversión.
  • Se observó una señal de fondo baja.
  • Inició con éxito el ensamblaje dinámico del ADN en respuesta a la unión a proteínas específicas.

Conclusiones:

  • La estrategia desarrollada permite el ensamblaje dinámico de ADN iniciado por proteínas.
  • Esto abre nuevas vías para el diagnóstico molecular basado en proteínas, imágenes y aplicaciones terapéuticas.