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Dipeptidyl peptidasa 4 es un receptor funcional para el coronavirus humano emergente-EMC

V Stalin Raj¹, Huihui Mou, Saskia L Smits

¹Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.

|March 15, 2013

Resumen

Los investigadores identificaron la dipeptidil peptidasa 4 (DPP4) como el receptor celular para el coronavirus humano-Centro Médico Erasmus (hCoV-EMC). Este descubrimiento ofrece información sobre el virus.

Área de la Ciencia:

Virología Virología.
Enfermedades infecciosas Enfermedades infecciosas.
Biología Molecular Biología Molecular

Sus antecedentes:

Los coronavirus humanos generalmente causan enfermedades respiratorias leves.
Los coronavirus emergentes como el SARS y el hCoV-EMC pueden causar infecciones graves y letales del tracto respiratorio inferior.
El hCoV-EMC comparte similitudes genéticas con los coronavirus del murciélago.

Objetivo del estudio:

Para identificar el receptor celular para el hCoV-EMC.
Comprender el rango de huéspedes y la patogénesis del hCoV-EMC.
Informar el desarrollo de estrategias de intervención.

Principales métodos:

Co-purificación de DPP4 con el dominio S1 de la proteína del pico del hCoV-EMC.
Inhibición de la infección por hCoV-EMC mediante el uso de anticuerpos anti-DPP4.
Expresión de DPP4 humano y de murciélago en células no susceptibles para probar la infectividad.

Principales resultados:

Dipeptidyl peptidasa 4 (DPP4) fue identificado como el receptor funcional para el hCoV-EMC.
DPP4 se une específicamente al dominio S1 de la proteína del pico del hCoV-EMC.
Los anticuerpos anti-DPP4 bloquearon la infección por el hCoV-EMC en cultivos celulares.
El DPP4 humano y de murciélago habilitó la infección por hCoV-EMC en células no permisivas.

Conclusiones:

DPP4 es un determinante clave de la entrada celular del hCoV-EMC y el tropismo del huésped.
La naturaleza conservada de DPP4 en todas las especies sugiere un potencial de amplio rango de huéspedes para el hCoV-EMC.
Comprender la interacción DPP4-hCoV-EMC es crucial para desarrollar terapias antivirales y predecir la propagación de la enfermedad.

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