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Una vía de biosíntesis de kanosamina no reconocida anteriormente en el Bacillus subtilis
Natasha D Vetter1, David M Langill, Shazia Anjum
1Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK, Canada, S7N 5C9.
Journal of the American Chemical Society
|April 17, 2013
Resumen
Las enzimas de Bacillus subtilis NtdA, NtdB y NtdC sintetizan la canosamina a partir del glucosa-6-fosfato. Este descubrimiento revela una nueva vía de biosíntesis de antibióticos, distinta de las rutas previamente conocidas.
Área de la Ciencia:
- Microbiología Microbiología.
- La bioquímica es la bioquímica.
- Enzimología Enzimología.
Sus antecedentes:
- El operón ntd en Bacillus subtilis es crucial para la síntesis de 3,3'-neotrehalosadiamina (NTD), un disacárido con propiedades antibióticas.
- Las funciones específicas de las enzimas NtdA, NtdB y NtdC dentro de este operón no se habían caracterizado previamente in vitro.
Objetivo del estudio:
- Para aclarar las funciones enzimáticas in vitro de NtdA, NtdB y NtdC de Bacillus subtilis.
- Caracterizar la nueva vía para la biosíntesis de la kanosamina en el Bacillus subtilis.
Principales métodos:
- Se realizaron ensayos enzimáticos para determinar las actividades catalíticas de NtdA, NtdB y NtdC purificados.
- Se analizaron la especificidad del sustrato y los productos de reacción para definir la vía bioquímica.
Principales resultados:
- NtdC fue identificado como una glucosa-6-fosfato 3-dehidrogenasa.
- La NtdA funciona como una 3-oxo-glucosa-6-fosfato:glutamato aminotransferasa dependiente del fosfato piridoxal.
- NtdB actúa como una canosamina-6-fosfato fosfatasa, completando la vía de biosíntesis de la canosamina a partir de la glucosa-6-fosfato.
Conclusiones:
- El estudio demuestra una nueva vía enzimática para la biosíntesis de la canosamina en Bacillus subtilis, catalizada por NtdA, NtdB y NtdC.
- Esta vía representa una vía alternativa para la producción de kanosamina en comparación con la vía dependiente de la UDP-glucosa que se encuentra en la Amycolatopsis mediterranei.
- Los hallazgos proporcionan nuevos conocimientos sobre la biosíntesis de azúcares inusuales con posibles aplicaciones antibióticas.
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