Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A TCER-1-siRNA Regulatory Axis Suppresses Antibacterial Innate Immunity in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Mutations in mitochondrial ferredoxin FDX2 suppress frataxin deficiency.

Nature·2025
Same author

From nematode to Nobel: How community-shared resources fueled the rise of <i>Caenorhabditis elegans</i> as a research organism.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Argonaute-siRNA loading via the RNA-binding protein RDE-4 in C. elegans.

Current biology : CB·2025
Same author

The microRNA <i>miR-243</i> directs poly(UG) modification of a somatic mRNA in <i>C. elegans</i>.

microPublication biology·2025
Same author

Hypoxia ameliorates neurodegeneration and movement disorder in a mouse model of Parkinson's disease.

Nature neuroscience·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: May 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Los microARN visitan el servicio de urgencias.

Taiowa A Montgomery1, Gary Ruvkun

  • 1Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.

Cell
|April 30, 2013
PubMed
Resumen
Este resumen es generado por máquina.

Ahora se sabe que los microARN de las plantas (miRNA) regulan objetivos génicos dentro del retículo endoplasmático. Este estudio identifica un nuevo componente de la vía de miRNA de la planta involucrada en el silenciamiento de genes.

Más Videos Relacionados

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Videos de Experimentos Relacionados

Last Updated: May 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Ciencias de las plantas Ciencias de las plantas.
  • Genética La genética.

Sus antecedentes:

  • Los microARN (miARN) son reguladores cruciales de la expresión génica en los eucariotas.
  • La localización subcelular precisa del silenciamiento de genes mediado por miRNA sigue siendo en gran medida desconocida para muchas vías.
  • Comprender la regulación de la diana del miRNA es clave para descifrar el control de la expresión génica.

Objetivo del estudio:

  • Para investigar la ubicación celular donde los microARN (miRNA) ejercen sus funciones reguladoras.
  • Para identificar nuevos componentes involucrados en la vía de miRNA de la planta.
  • Para dilucidar el papel de orgánulos específicos en el silenciamiento de genes mediado por miRNA.

Principales métodos:

  • Utilizó técnicas avanzadas de biología molecular para estudiar la actividad del miRNA en las plantas.
  • Investigó la interacción de los componentes de la vía del miRNA con los orgánulos celulares.
  • Se emplean ensayos de silenciamiento de genes para confirmar las funciones funcionales.

Principales resultados:

  • Se identificó una nueva proteína involucrada en la vía del miRNA vegetal.
  • Se demostró que el retículo endoplasmático es un sitio para el silenciamiento de genes mediado por miRNA.
  • Proporcionó evidencia de la importancia funcional del retículo endoplasmático en este proceso.

Conclusiones:

  • El retículo endoplasmático juega un papel importante en el silenciamiento de genes mediado por miRNA de las plantas.
  • Este hallazgo amplía nuestra comprensión de la localización subcelular de la actividad del miRNA.
  • Abre nuevas vías para la investigación de la regulación genética post-transcripcional en las plantas.