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Las robustas oscilaciones circadianas en el crecimiento de las cianobacterias requieren retroalimentación

Shu-Wen Teng¹, Shankar Mukherji, Jeffrey R Moffitt

¹Howard Hughes Medical Institute, Faculty of Arts and Sciences Center for Systems Biology, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)

|May 11, 2013

Resumen

Los ritmos circadianos de las cianobacterias dependen de la regulación posttranslacional (PTR) para las oscilaciones. La regulación de retroalimentación transcripcional-traducional (TTR) es crucial para mantener la estabilidad del oscilador individual y la sincronía de la población en estos organismos.

Área de la Ciencia:

* Biología molecular.
* Biología de sistemas * Biología de sistemas
* Cronobiología * Cronobiología

Sus antecedentes:

* Los ritmos circadianos son procesos biológicos endógenos que regulan los ciclos diarios.
* Las cianobacterias exhiben robustas oscilaciones circadianas, cruciales para los procesos celulares.
* El reloj circadiano de las cianobacterias implica complejos mecanismos reguladores.

Objetivo del estudio:

* Para investigar la suficiencia del circuito de regulación post-traducional (PTR) en la generación de oscilaciones circadianas en las cianobacterias.
* Determinar el papel de la regulación de retroalimentación transcripcional-traducional (TTR) en el mantenimiento de la estabilidad del oscilador y la sincronía de la población.
* Para modelar el reloj circadiano cianobacteriano para comprender la interacción entre PTR y TTR.

Principales métodos:

* Análisis experimental de componentes del reloj circadiano de cianobacterias.
* Modelado matemático de los circuitos reguladores circadianos.
* Investigación de la estabilidad del oscilador y la sincronía de la población bajo diferentes condiciones regulatorias.

Principales resultados:

* El circuito de regulación posttranslacional (PTR) por sí solo es suficiente para generar oscilaciones circadianas en las cianobacterias en crecimiento.
* En ausencia de regulación de retroalimentación transcripcional-traducional (TTR), los osciladores individuales mostraron una estabilidad reducida.
* La sincronía de la población no se mantuvo en las cianobacterias que carecían de TTR, como lo demuestran los datos experimentales y el modelado.
* El modelado matemático confirmó las oscilaciones sostenidas en el circuito PTR y destacó la importancia de TTR para la sincronización.

Conclusiones:

* El circuito de regulación postraslacional (PTR) es un componente central del reloj circadiano de las cianobacterias.
* La regulación de retroalimentación transcripcional-traducional (TTR, por sus siglas en inglés) juega un papel vital en la estabilización de los osciladores circadianos individuales y en asegurar la sincronía de la población.
* Una combinación de PTR y TTR asegura un comportamiento circadiano robusto y sincronizado en las cianobacterias.

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