Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Unhealthy air quality secondary to wildfires is associated with lower blastocyst yield.

Fertility and sterility·2024
Same author

Utility of Intraprocedural Luminal Diameter and Distensibility Measurements During the Esophageal Peroral Endoscopic Myotomy Procedure.

Journal of clinical gastroenterology·2024
Same author

Locoregional Therapies for Hepatocellular Carcinoma prior to Liver Transplant: Comparative Pathologic Necrosis, Radiologic Response, and Recurrence.

Journal of vascular and interventional radiology : JVIR·2023
Same author

Social Media Use and Its Concurrent and Subsequent Relation to a Biological Marker of Inflammation: Short-Term Longitudinal Study.

Journal of medical Internet research·2023
Same author

Metabolic tagging of extracellular vesicles and development of enhanced extracellular vesicle based cancer vaccines.

Nature communications·2023
Same author

Endoscopic Nutrition of Patients with Cancer.

Gastrointestinal endoscopy clinics of North America·2023

Video Experimental Relacionado

Updated: May 10, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Células madre embrionarias humanas derivadas por transferencia de núcleos de células somáticas.

Masahito Tachibana1, Paula Amato, Michelle Sparman

  • 1Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.

Cell
|May 21, 2013
PubMed
Resumen

Los investigadores crearon con éxito células madre embrionarias de transferencia nuclear humana (NT-ESC) mediante la optimización de la transferencia nuclear de células somáticas (SCNT). Este avance supera los desafíos anteriores en el desarrollo embrionario temprano, allanando el camino para la medicina regenerativa emparejada con el paciente.

Más Videos Relacionados

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: October 1, 2010

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 24, 2014

Videos de Experimentos Relacionados

Last Updated: May 10, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: October 1, 2010

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 24, 2014

Área de la Ciencia:

  • Biología de las células madre Biología de las células madre
  • La Medicina Reproductiva es una de ellas.
  • Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

  • La transferencia nuclear de células somáticas (SCNT, por sus siglas en inglés) ofrece una ruta a transferencia nuclear (NT, por sus siglas en inglés) -ESCs emparejadas con pacientes para la investigación y las terapias de enfermedades.
  • Los esfuerzos previos para obtener NT-ESC humanas se vieron obstaculizados por la detención temprana de embriones SCNT.

Objetivo del estudio:

  • Identificar y superar los factores clave que limitan la derivación humana de NT-ESC a través de SCNT.
  • Establecer un método confiable para generar NT-ESCs humanos compatibles con el paciente.

Principales métodos:

  • Identificó la salida prematura de la meiosis en los ovocitos y la activación subóptima como barreras críticas.
  • Desarrolló protocolos SCNT optimizados para abordar estas limitaciones.
  • Se aplicó SCNT optimizada a ovocitos humanos de alta calidad.

Principales resultados:

  • Se obtuvieron con éxito líneas humanas NT-ESC utilizando SCNT optimizada, incluso a partir de un número limitado de ovocitos (tan sólo dos).
  • Las NT-ESC derivadas exhibieron cariotipos diploides normales y heredaron exclusivamente el genoma nuclear de las células somáticas del donante.
  • Las NT-ESC demostraron patrones de expresión génica y diferenciación comparables a las ESC de origen embrionario, lo que indica una reprogramación exitosa.

Conclusiones:

  • Los protocolos SCNT optimizados superan las barreras anteriores para la derivación humana de NT-ESC.
  • Este método permite la generación de células madre pluripotentes emparejadas con el paciente para aplicaciones terapéuticas y de investigación.
  • La reprogramación eficiente de las células somáticas a la pluripotencia se logró a través de SCNT optimizada.