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Catálisis de ADN de una reacción de hidrólisis de ARN normalmente desfavorecida

Darren J Parker¹, Ying Xiao, John M Aguilar

¹Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society

|May 24, 2013

Resumen

Se pueden seleccionar desoxiribozimas de ADN para dividir el ARN a través de la hidrólisis, una vía desfavorecida. Esto amplía la comprensión de la catálisis del ADN más allá de las reacciones de aceleración.

Área de la Ciencia:

La bioquímica es la bioquímica.
Biología Molecular Biología Molecular
La catálisis de la catálisis.

Sus antecedentes:

Las desoxiribozimas suelen catalizar la hidrólisis del ADN o la transesterificación del ARN.
La escisión del ARN por hidrólisis es generalmente desfavorable en comparación con la transesterificación.

Objetivo del estudio:

Para investigar la capacidad del ADN para catalizar la escisión del ARN a través de la hidrólisis.
Para determinar si se pueden seleccionar desoxirribozimas para una vía de reacción desfavorecida.

Principales métodos:

Se emplearon experimentos de selección in vitro.
Se realizó la re-selección de los desoxiribozimas existentes y la selección de novo de pools de ADN aleatorios.
Se aplicó una estricta presión de selección para la hidrólisis.

Principales resultados:

Se podrían seleccionar desoxiribozimas para catalizar la hidrólisis del ARN.
La presión de selección determinó si se había producido hidrólisis o transesterificación.
Se identificaron nuevas desoxiribozimas para la hidrólisis del ARN de un grupo aleatorio.

Conclusiones:

Pequeñas secuencias de ADN pueden dirigir la catálisis por una vía desfavorable (hidrólisis).
Esto expande las conocidas capacidades catalíticas del ADN más allá de la aceleración de la velocidad.
La catálisis del ADN es más versátil de lo que se entendía anteriormente.

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