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Un cambio entre dominios topológicos subyace a la colinealidad de los genes HoxD en las extremidades del ratón
Guillaume Andrey1, Thomas Montavon, Bénédicte Mascrez
1School of Life Sciences, Federal Institute of Technology, Lausanne, 1015 Lausanne, Switzerland.
Science (New York, N.Y.)
|June 8, 2013
Resumen
La regulación del gen Hoxd en las extremidades del ratón cambia entre dos dominios durante el desarrollo. Este interruptor es crucial para la formación de las estructuras de la muñeca y los dedos, lo que explica la organización genética colineal en los apéndices de los vertebrados.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Los genes Hox son críticos para establecer el plan corporal del animal a lo largo del tronco y los ejes apendiculares.
- Durante el desarrollo de las extremidades del ratón, los genes Hoxd exhiben dos ondas transcripcionales distintas: temprana (especificación del brazo / antebrazo) y tardía (formación de dígitos).
Objetivo del estudio:
- Investigar el mecanismo regulador subyacente a la transición entre la expresión génica temprana y tardía de Hoxd durante el desarrollo de las extremidades.
- Comprender cómo el cambio de dominio topológico influye en la regulación del gen Hox y el patrón de las extremidades.
Principales métodos:
- Análisis de los patrones de transcripción del gen Hoxd durante el desarrollo de las extremidades del ratón.
- Examen de las interacciones de dominio topológico dentro del grupo de genes Hox.
Principales resultados:
- Un cambio funcional entre dominios topológicos opuestos ocurre durante el desarrollo de las extremidades.
- La transición de los genes Hoxd centrales de las interacciones del dominio telomérico al dominio centromérico.
- Este interruptor facilita la formación de la región de la muñeca y explica la regulación genética colineal.
Conclusiones:
- El cambio regulatorio entre dominios topológicos pone de relieve la modularidad de las extremidades y la divergencia evolutiva.
- Este mecanismo explica el preciso control espacial y temporal de la expresión del gen Hox en los apéndices de los vertebrados.
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