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Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Los mastocitos como fuente de TNF-alfa/cachectina preformada e inmunológicamente inducible

J R Gordon¹, S J Galli

¹Department of Pathology, Beth Israel Hospital, Boston, Massachusetts 02215.

|July 19, 1990

Resumen

Los mastocitos de ratón producen y liberan constitutivamente el factor de necrosis tumoral alfa (TNF-alfa). La activación dependiente de IgE mejora esta liberación, identificando a los mastocitos como una fuente clave de TNF-alfa involucrada en la defensa del huésped y las enfermedades alérgicas.

Área de la Ciencia:

Inmunología Inmunología.
Biología celular Biología celular.
Investigación de citoquinas Investigación de citoquinas.

Sus antecedentes:

El factor de necrosis tumoral alfa (TNF-alfa) es una citocina clave involucrada en la inflamación, la inmunidad y el metabolismo.
Si bien los macrófagos son productores conocidos, otros tipos de células, incluidos los mastocitos, también pueden producir TNF-alfa.
Estudios previos sugirieron que los mastocitos podrían contener productos similares al TNF-alfa.

Objetivo del estudio:

Para investigar la presencia y regulación de la bioactividad del TNF-alfa en los mastocitos de ratón.
Para determinar si los mastocitos liberan TNF-alfa después de la activación.
Para identificar los mastocitos como una fuente significativa de TNF-alfa.

Principales métodos:

Análisis de la bioactividad del TNF-alfa en mastocitos peritoneos de ratones residentes y en cultivo.
La activación dependiente de IgE de los mastocitos.
Medición del ARN mensajero TNF-alfa (ARNm) y los niveles de bioactividad después de la activación.

Principales resultados:

Los mastocitos peritoneos de ratón residente constitutivamente contienen una sustancial bioactividad TNF-alfa.
Los mastocitos inmaduros cultivados mostraron un contenido variable de TNF-alfa.
La activación dependiente de IgE condujo a la liberación extracelular de TNF-alfa y aumentó el ARNm TNF-alfa y la bioactividad.

Conclusiones:

Los mastocitos de ratón son una fuente significativa de TNF-alfa. tanto preformado como inducible.
La liberación de TNF-alfa derivada de las células de mastocitos puede desempeñar un papel en los mecanismos de defensa del huésped.
El TNF-alfa de mastocitos contribuye a la fisiopatología de las enfermedades alérgicas y otros procesos mediados por el TNF-alfa.

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