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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
Una sola gD glicoproteína puede mediar la infección por el virus del herpes simple
Richard W Clarke1, Anna Drews, Helena Browne
1Chemistry Department, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. rwc25@cam.ac.uk
Journal of the American Chemical Society
|July 11, 2013
Resumen
La entrada del virus del herpes simple (HSV) en las células es altamente eficiente. Una única glicoproteína D (gD) no unida en el virión del HSV es suficiente para la entrada en la célula, incluso con cientos de gD presentes.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Inmunología Inmunología.
Sus antecedentes:
- Los virus del herpes simple (HSV) poseen numerosas moléculas de glicoproteína D (gD) en su superficie.
- La neutralización del HSV generalmente requiere una gran cantidad de anticuerpos por virión.
Objetivo del estudio:
- Determinar el número mínimo de glicoproteínas gD libres requeridas para la infectividad por virus HSV de tipo silvestre.
- Para investigar la eficiencia de la entrada de HSV a nivel molecular.
Principales métodos:
- Análisis cuantitativo de las partículas de HSV etiquetadas con fluorescencia.
- Los ensayos de neutralización del virus utilizan anticuerpos monoclonales dirigidos a la DG.
Principales resultados:
- Un virión HSV de tipo salvaje requiere solo una o dos glicoproteínas gD no unidas para infectar una célula.
- Esta infectividad se mantiene a pesar de la presencia de aproximadamente 300 gD de glicoproteínas en el virión.
Conclusiones:
- La entrada de HSV es un proceso excepcionalmente eficiente, que opera a nivel de complejos moleculares individuales.
- Los hallazgos desafían las suposiciones anteriores sobre los requisitos de anticuerpos para la neutralización del HSV.
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