Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight Junctions
Tight...
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight Junctions
Tight...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Morphological control of gut lumen access shapes enteroendoceine cell function.

The Journal of cell biology·2026
Same author

TS3 Asn347 corticosteroid-binding globulin (CBG) glycoform deficiency is associated with mortality in human septic shock.

Journal of the Endocrine Society·2026
Same author

Deciphering mechanical determinants of morphological evolution.

Cell·2026
Same author

Epithelial cell plasticity in metazoans: Evolutionary insights into roles and mechanisms.

Seminars in cell & developmental biology·2026
Same author

Proteomic analysis of FACS-enriched whole nematocysts from the colonial hydrozoan <i>Hydractinia symbiolongicarpus</i>.

Toxicon: X·2026
Same author

Loss of SPECC1L in cranial neural crest cells results in increased hedgehog signaling and frontonasal dysplasia.

Frontiers in physiology·2026

Video Experimental Relacionado

Updated: May 9, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

Las uniones epiteliales mantienen la arquitectura del tejido dirigiendo la orientación del huso plano.

Yu-ichiro Nakajima1, Emily J Meyer, Amanda Kroesen

  • 1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA.

Nature
|July 23, 2013
PubMed
Resumen

La alineación adecuada del huso mitótico es crucial para mantener la integridad del tejido epitelial. Las interrupciones pueden conducir al cáncer, pero las proteínas de unión como Scribbled y Discs large 1 ayudan a regular este proceso.

Más Videos Relacionados

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Videos de Experimentos Relacionados

Last Updated: May 9, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Área de la Ciencia:

  • Biología celular Biología celular.
  • Biología del desarrollo Biología del desarrollo.
  • Investigación de la investigación del cáncer.

Sus antecedentes:

  • La proliferación de células epiteliales requiere la alineación del huso plano para la arquitectura del tejido.
  • La alineación interrumpida del husillo está relacionada con la transición epitelial a mesenquimal y el cáncer.
  • Los mecanismos in vivo que regulan la orientación del husillo no se comprenden completamente.

Objetivo del estudio:

  • Para investigar los mecanismos in vivo de la alineación del huso plano en los tejidos epiteliales.
  • Identificar los reguladores clave de la orientación del huso mitótico y la integridad epitelial.

Principales métodos:

  • Utilizó los discos imaginarios de Drosophila como un sistema modelo.
  • Investigó las funciones de la corteza de actomyosina y las proteínas de unión (Scribbled, Discs large 1).
  • Efectos observados de las desalineaciones del husillo en la delaminación celular, la apoptosis y la formación de tumores.

Principales resultados:

  • La corteza de actomiosina y la unión Scribbled / Discos grandes 1 son esenciales para la alineación del huso plano.
  • La alineación defectuosa del husillo se correlaciona con la delaminación celular y la apoptosis.
  • La inhibición de la apoptosis en las células desalineadas promueve la formación de masa basal parecida a un tumor.

Conclusiones:

  • La alineación del husillo mediada por la unión es vital para mantener la integridad epitelial.
  • La polaridad epitelial y los mecanismos de muerte celular poseen funciones inherentes supresoras de tumores.