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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
Un atlas de la composición de RNP en todo el transcriptoma revela diversas clases de mRNA y lncRNA
Alex Charles Tuck1, David Tollervey1
1The University of Edinburgh, Wellcome Trust Centre for Cell Biology, Michael Swann Building, Kings Buildings, Edinburgh EH9 3JR, UK.
Cell
|September 3, 2013
Resumen
Los ARN mensajeros (ARNm) y los ARN largos no codificantes (ARNlnc) comparten pasos de procesamiento, pero difieren en su destino. El destino de la transcripción está determinado por la formación del extremo 3', con la mayoría de los lncRNA sometidos a vigilancia nuclear.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- ARN Biología Biología ARN
Sus antecedentes:
- Los genomas eucariotas producen diversos ARN mensajeros (ARNm) y ARN largos no codificantes (ARNlnc).
- Ambos tipos de ARN son transcritos por la ARN polimerasa II y se someten a 5' capping y 3' poliadenilación.
- Una distinción clave radica en la traducción de ARNm a proteínas, mientras que los ARNm lnc no codifican proteínas.
Objetivo del estudio:
- Para investigar los mecanismos moleculares que distinguen el mRNA y el lncRNA.
- Para identificar objetivos de todo el transcriptoma de los factores clave de procesamiento, exportación y facturación de ARN en levaduras.
- Para entender cómo las vías de maduración de la transcripción divergen entre los ARNm y los ARNm lnc.
Principales métodos:
- Análisis de todo el transcriptoma de los factores de procesamiento de ARN en la levadura en ciernes.
- Análisis comparativo de las vías de maduración del ARNm y el ARNn.
- Identificación de los factores que influyen en la formación del extremo 3' y la exportación nuclear.
Principales resultados:
- El destino de la transcripción se determina principalmente durante el procesamiento final del 3'.
- La mayoría de los lncRNA se dirigen a las vías de vigilancia del ARN nuclear.
- Un subconjunto de lncRNAs con extremos 3' similares al ARNm se exporta al citoplasma.
- Las proteínas Hrp1 y Nab2 juegan un doble papel en el procesamiento del ARNm y la vigilancia del lncRNA.
- Un subconjunto de ARNm exhiben características "similares a lncRNA" y están regulados por la vigilancia nuclear.
Conclusiones:
- La formación del extremo 3' es un punto de control crítico para la determinación del destino del ARNm vs. lncRNA.
- Los factores de procesamiento de ARN como Hrp1 y Nab2 orquestan las decisiones de destino de la transcripción.
- La maquinaria de vigilancia nuclear también tiene un impacto en la maduración y exportación de ciertos ARNm.
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