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La decisión de proliferación-quiescencia está controlada por una bifurcación en la actividad de CDK2 en la salida

Sabrina L Spencer¹, Steven D Cappell, Feng-Chiao Tsai

¹Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.

|October 1, 2013

Resumen

La proliferación celular está controlada por la actividad de la cinasa dependiente de ciclina (CDK). Un nuevo sensor revela que las células deciden después de la mitosis si proliferan o entran en quiescencia, reguladas por p21 y mitógenos.

Área de la Ciencia:

Biología celular Biología celular.
Biología molecular La biología molecular.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

La homeostasis del tejido depende de las transiciones del ciclo celular entre la quiescencia y la proliferación.
La actividad de la cinasa dependiente de ciclina (CDK) impulsa la progresión del ciclo celular en las células proliferantes.

Objetivo del estudio:

Desarrollar un sensor de células vivas para la actividad de la cinasa 2 dependiente de ciclina (CDK2).
Para investigar las decisiones del destino celular inmediatamente después de la mitosis.

Principales métodos:

Desarrollo de un nuevo sensor de células vivas para monitorear la actividad de CDK2.
Análisis de las poblaciones celulares que salen de la mitosis.
Investigación del papel de p21 y mitógenos en la regulación del ciclo celular.

Principales resultados:

Se observó una bifurcación en las poblaciones celulares que salían de la mitosis basada en la actividad de CDK2.
Las células entran inmediatamente en el siguiente ciclo celular o entran en un estado transitorio de quiescencia.
Esta decisión está controlada por el inhibidor de CDK p21 e influenciada por mitógenos durante una ventana específica del ciclo celular.

Conclusiones:

Las células toman una decisión crítica al final de la mitosis con respecto a la entrada en el ciclo celular o la quiescencia.
La dinámica de la actividad de CDK2, regulada por p21 y mitógenos, dicta este destino celular.
Esto proporciona nuevos conocimientos sobre la regulación de la proliferación celular y la homeostasis de los tejidos.

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