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La glucólisis sintética no oxidativa permite la conservación completa del carbono

Igor W Bogorad¹, Tzu-Shyang Lin, James C Liao

¹1] Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA. [2] Departments of Bioengineering and of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA.

|October 1, 2013

Resumen

Desarrollamos la glicólisis no oxidativa (NOG), una nueva vía metabólica que previene la pérdida de carbono de los azúcares. Esta innovación permite la conservación completa del carbono para la producción de productos químicos y combustibles valiosos.

Área de la Ciencia:

Ingeniería Metabólica Ingeniería Metabólica.
Biología sintética Biología sintética.
La bioquímica es la bioquímica.

Sus antecedentes:

La glicólisis es una vía metabólica fundamental para la descomposición del azúcar en la mayoría de los organismos.
La vía glicolítica nativa da lugar a la pérdida de carbono durante la descarboxilación del piruvato, lo que limita el rendimiento de valiosos metabolitos de C2.
Esta pérdida de carbono es un desafío significativo en la biorefinación y la producción microbiana.

Objetivo del estudio:

Diseñar y construir una nueva vía metabólica para la conservación completa de carbono durante el catabolismo del azúcar.
Para eliminar la pérdida de carbono asociada con la descarboxilación de piruvato en la glicólisis.
Para permitir la producción estequiométrica de metabolitos de C2 a partir de varios fosfatos de azúcar.

Principales métodos:

Diseñado y construido una vía metabólica cíclica no oxidativa (NOG).
Se probó la vía de NOG in vitro e in vivo utilizando Escherichia coli.
Rendimiento de carbono evaluado y eficiencia de producción de metabolitos.

Principales resultados:

La vía NOG permite la producción estequiométrica de metabolitos de C2 a partir de hexosa, pentosa y fosfatos de triosa sin pérdida de carbono.
Se ha demostrado la conservación completa del carbono en el catabolismo del azúcar al acetil-CoA.
Mostró el potencial del NOG para la producción de alto rendimiento de combustibles y productos químicos cuando se combina con las vías de asimilación C1.

Conclusiones:

La glucólisis no oxidativa (NOG) ofrece una solución a la pérdida de carbono en el metabolismo del azúcar.
El NOG permite un rendimiento de carbono del 100% para producir compuestos valiosos a partir de azúcares.
Esta vía tiene implicaciones significativas para el biorefinado sostenible y la síntesis química.

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