Video Experimental Relacionado
Updated: May 7, 2026
10:19
Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
Los pequeños ARN de los hongos suprimen la inmunidad de las plantas secuestrando las vías de interferencia del ARN
Arne Weiberg1, Ming Wang, Feng-Mao Lin
1Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA.
Science (New York, N.Y.)
|October 5, 2013
Resumen
Botrytis cinerea utiliza ARN pequeños (Bc-sRNAs) para suprimir los genes de inmunidad de las plantas, lo que permite la enfermedad del moho gris. Esta interferencia de ARN entre reinos pone de relieve una avanzada estrategia de virulencia fúngica.
Área de la Ciencia:
- Patología Vegetal Patología vegetal.
- Biología Molecular Biología Molecular
- La micología de la micología.
Sus antecedentes:
- Botrytis cinerea causa la enfermedad del moho gris en más de 200 especies de plantas.
- Los patógenos fúngicos a menudo emplean mecanismos sofisticados para superar las defensas del huésped.
Objetivo del estudio:
- Para investigar el papel de Botrytis cinerea pequeños ARN (Bc-sRNAs) en la inmunidad de las plantas.
- Para dilucidar el mecanismo por el cual los Bc-sRNAs interactúan con las vías de interferencia del ARN huésped (RNAi).
Principales métodos:
- Análisis de Bc-sRNAs dirigidos a los genes de inmunidad de las plantas.
- Utilizando mutantes de Arabidopsis ago1 para evaluar la susceptibilidad.
- El uso de mutantes dobles de Botrytis cinerea dcl1 dcl2 para evaluar la patogenicidad.
Principales resultados:
- Se descubrió que los ARNs Bc-s específicos silencian los genes de inmunidad de la Arabidopsis y el tomate.
- Bc-sRNAs secuestran la maquinaria de interferencia de ARN del huésped al interactuar con Argonauta 1 (AGO1).
- Los mutantes de Arabidopsis ago1 mostraron una menor susceptibilidad a B. cinerea, y los mutantes de dcl1 y dcl2 mostraron una menor patogenicidad.
Conclusiones:
- Botrytis cinerea emplea pequeños efectores de ARN virulentos para suprimir la inmunidad del huésped.
- La interferencia de ARN cruzado de reino que ocurre naturalmente es un mecanismo clave de virulencia para B. cinerea.
- Este estudio revela una estrategia avanzada para la patogénesis fúngica y la supresión inmune del huésped.
Videos de Conceptos Relacionados
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...