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Hidrólisis catalizada por ADN de ésteres y amidas aromáticas

Benjamin M Brandsen¹, Anthony R Hesser, Marissa A Castner

¹Department of Chemistry, University of Illinois at Urbana-Champaign , 600 South Mathews Avenue, Urbana, Illinois 61801, United States.

Journal of the American Chemical Society

|October 17, 2013

Resumen

Los catalizadores de ADN (deoxirribozimas) ahora pueden hidrolizar los enlaces de éster y amida aromática, expandiendo sus capacidades catalíticas más allá del ADN. Este avance abre caminos para nuevas investigaciones de catálisis basadas en el ADN.

Área de la Ciencia:

La bioquímica es la bioquímica.
Biología Molecular Biología Molecular
La catálisis de la catálisis.

Sus antecedentes:

Anteriormente se sabía que los catalizadores de ADN (desoxirribozimas) podían cortar los enlaces de fosfodiéster del ADN.
La hidrólisis catalizada por ADN de los enlaces amídicos siguió siendo un desafío difícil de alcanzar en la investigación bioquímica.

Objetivo del estudio:

Desarrollar catalizadores de ADN capaces de hidrolizar los enlaces de éster y amida aromática.
Para investigar el mecanismo de la hidrólisis de amida aromática catalizada por el ADN.

Principales métodos:

Se empleó la selección in vitro para identificar nuevos desoxirribozimas.
El análisis de la relación de energía libre lineal se utilizó para estudiar el mecanismo de hidrólisis.

Principales resultados:

Identificaron con éxito catalizadores de ADN que hidrolizan los enlaces de éster.
Desarrolló catalizadores de ADN que hidrolizan amidas aromáticas con un grupo de salida de anilina.
El análisis sugiere un mecanismo general de eliminación catalizado por ácido para la hidrólisis de amida aromática.

Conclusiones:

El ADN posee actividad catalítica para la hidrólisis de ésteres y amidas aromáticas.
Los hallazgos proporcionan una base para el desarrollo de catalizadores de ADN para la hidrólisis de amida alifática.

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