Video Experimental Relacionado
Updated: May 6, 2026
09:28
In Vitro Transcription Assays and Their Application in Drug Discovery
Published on: September 20, 2016
14.5K
El factor de transcripción dependiente de la actividad NPAS4 regula la inhibición específica del dominio
Brenda L Bloodgood1, Nikhil Sharma, Heidi Adlman Browne
11] Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA [3].
Nature
|November 9, 2013
Resumen
La expresión de NPAS4 impulsada por el comportamiento en ratones reorganiza las sinapsis inhibidoras en las neuronas piramidal CA1. Esta regulación aumenta la inhibición somática mientras disminuye la inhibición dendrítica, lo que afecta la producción neuronal y la plasticidad.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología Molecular Biología Molecular
- La neurociencia computacional es una neurociencia computacional.
Sus antecedentes:
- Las neuronas inhibidoras regulan el flujo de información del circuito neuronal a través de sinapsis en las neuronas piramidal.
- La inhibición somática y dendrítica controla diferencialmente la excitabilidad y plasticidad neuronales.
- NPAS4 (NPAS4) es un factor de transcripción dependiente de la actividad que regula el número y la función de las sinapsis inhibidoras.
Objetivo del estudio:
- Para investigar el papel in vivo de NPAS4 en la coordinación de la distribución de sinapsis inhibidoras en distintos dominios neuronales.
- Para dilucidar cómo NPAS4 afecta la inhibición somática versus dendrítica en las neuronas piramidal CA1 del hipocampo.
Principales métodos:
- Se utilizaron modelos de ratón para estudiar la función de NPAS4 en el hipocampo.
- Analizó la expresión de NPAS4 impulsada por el comportamiento y su impacto en la distribución de la sinapsis inhibidora.
- Investigó el papel de los genes diana NPAS4, como el factor neurotrófico derivado del cerebro (BDNF), en la mediación de estos efectos.
Principales resultados:
- La expresión de NPAS4 impulsada por el comportamiento indujo una redistribución de las sinapsis inhibidoras en las neuronas piramidal CA1.
- NPAS4 aumentó el número de sinapsis inhibitorias en el cuerpo celular (soma) mientras que lo disminuyó en las dendritas apicales.
- El gen blanco NPAS4, BDNF, fue identificado como un mediador, específicamente regulando la inhibición somática.
Conclusiones:
- Los estímulos sensoriales, a través de la inducción de NPAS4, controlan diferencialmente las características espaciales de la inhibición neuronal.
- Esta reorganización espacial restringe la salida neuronal al mejorar la inhibición somática.
- Los hallazgos sugieren que NPAS4 crea un entorno dendrítico permisivo para la plasticidad mediante la modulación de la inhibición.
Videos de Conceptos Relacionados
01:52Eukaryotic Transcription Inhibitors
9.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.1K
02:04Co-activators and Co-repressors
7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
02:36RNA Polymerase II Accessory Proteins
8.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
8.9K
01:20The JAK-STAT Signaling Pathway
10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.2K
02:26NF-κB-dependent Signaling Pathway
7.7K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.7K
02:23Master Transcription Regulators
6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K