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Los inteínos divididos naturalmente se ensamblan a través de un mecanismo de "captura y colapso"

Neel H Shah¹, Ertan Eryilmaz, David Cowburn

¹Department of Chemistry, Princeton University , Frick Laboratory, Princeton, New Jersey 08544, United States.

Journal of the American Chemical Society

|November 19, 2013

Resumen

Los inteínos divididos logran su estructura única a través de un nuevo mecanismo en el que los fragmentos desordenados se unen y forman un intermedio ordenado antes de plegarse. Este hallazgo avanza en las tecnologías de ingeniería de proteínas.

Área de la Ciencia:

La bioquímica es la bioquímica.
Ingeniería de proteínas Ingeniería de proteínas.
Biología Molecular Biología Molecular

Sus antecedentes:

Las inteínas divididas son proteínas que realizan el empalme de proteínas en trans.
Su reconocimiento molecular y el mecanismo de ensamblaje siguen sin estar claros.
La química de las proteínas divididas es crucial para la ingeniería de proteínas.

Objetivo del estudio:

Para dilucidar el mecanismo de la división intein fragmento asociación y ensamblaje.
Para entender cómo se logra la topología única en la unión.
Proporcionar perspectivas para el avance de tecnologías basadas en split intein.

Principales métodos:

Técnicas biofísicas Las técnicas biofísicas.
Métodos de ingeniería de proteínas métodos de ingeniería de proteínas.
Etiquetado isotópico segmentario para el etiquetado isotópico.

Principales resultados:

Un fragmento intein dividido está parcialmente plegado, el otro está desordenado.
Las regiones desordenadas median la unión y forman un intermedio ordenado.
Este intermedio luego se colapsa en el pliegue intein nativo.

Conclusiones:

Se ha identificado un nuevo mecanismo para el ensamblaje dividido de proteínas que involucra regiones desordenadas.
Comprender este mecanismo mejora el conocimiento del plegamiento de proteínas y el reconocimiento molecular.
Esta investigación mejorará el desarrollo y la aplicación de tecnologías split intein.

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