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Un oncogén activado de Harvey ras produce tumores benignos en el tejido epidérmico del ratón
Nature
|October 5, 1986
Resumen
La introducción de un oncogén ras mutado en las células de la piel del ratón puede causar papilomas. Esto sugiere que el gen ras es un objetivo clave en la carcinogénesis de la piel, iniciando potencialmente la formación de tumores.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La carcinogénesis de la piel implica múltiples eventos genéticos, con el gen ras implicado como un objetivo temprano.
- El gen c-rasH activado que se encuentra en los papilomas cutáneos sugiere su papel en el inicio de mutaciones.
- El virus del sarcoma murino de Harvey (Ha-MSV) y el gen v-rasH pueden alterar la diferenciación de los queratinocitos in vitro.
Objetivo del estudio:
- Para investigar el papel del gen Ha-MSV v-rasH en la inducción de tumores de piel.
- Para determinar si la introducción de un oncogén ras mutado en los queratinocitos puede causar la formación de papilomas in vivo.
- Examinar la regulación transcripcional del oncogén exógeno en los tejidos tumorales.
Principales métodos:
- Introducción del gen Ha-MSV v-rasH en queratinocitos cultivados utilizando un vector retroviral defectuoso.
- Construcción de injertos de piel utilizando queratinocitos genéticamente modificados.
- Injerto en receptores de ratones desnudos atímicos para evaluar la formación de tumores.
- Análisis de la expresión oncogénica en tejidos tumorales diferenciados.
Principales resultados:
- Los injertos de piel de queratinocitos portadores del oncogén ras mutado produjeron papilomas en receptores de ratones.
- Se observó que la expresión del oncogén v-rasH introducido está regulada por la transcripción.
- La regulación se produjo en las regiones diferenciadas de los tumores benignos resultantes.
Conclusiones:
- El oncogén ras mutado puede inducir la formación de papilomas en la piel del ratón, apoyando su papel en el inicio de la carcinogénesis de la piel.
- La regulación transcripcional del oncogén exógeno en los tejidos tumorales diferenciados sugiere una compleja interacción entre la expresión oncogénica y la diferenciación.
- Este estudio proporciona evidencia in vivo del potencial oncogénico del gen ras en el desarrollo del cáncer de piel.
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