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Un nuevo método de etiquetado de la pared celular metabólica revela el peptidoglicano en Chlamydia trachomatis
G W Liechti1, E Kuru2, E Hall3
11] Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4799, USA [2].
Nature
|December 17, 2013
Resumen
Este estudio proporciona una fuerte evidencia de que las especies de Chlamydia poseen peptidoglicano funcional (PG), resolviendo el problema de larga data.
Área de la Ciencia:
- Microbiología Microbiología.
- Síntesis de la pared celular bacteriana.
- Enfermedades infecciosas Enfermedades infecciosas.
Sus antecedentes:
- El peptidoglicano (PG) es esencial para la estructura y división celular bacteriana.
- La clamidiales se consideró una excepción debido a la incapacidad de detectar la PG, conocida como la "anomalía clamidial".
- Las especies de clamidia poseen genes para la biosíntesis de PG y son susceptibles a los antibióticos dirigidos a PG.
Objetivo del estudio:
- Para investigar la presencia y la función del peptidoglicano en las especies de Chlamydia.
- Para resolver la "anomalía clamidial" de hace 50 años con respecto a PG.
- Proporcionar la evidencia más sólida hasta la fecha para la PG funcional en Chlamydia.
Principales métodos:
- Etiquetado metabólico del chlamydial PG utilizando nuevas sondas de dipeptido de ácido d-amino.
- Aplicación de la química del clic para la detección de PG.
- Experimentos de incorporación de sonda diferencial en presencia de ampicilina durante el ciclo de desarrollo de Chlamydia trachomatis.
Principales resultados:
- Se logró el etiquetado metabólico exitoso de la PG clamidial.
- La incorporación diferencial de la sonda indicó la presencia de enzimas modificadoras de PG.
- Los resultados son consistentes con la existencia de PG funcional en Chlamydia trachomatis.
Conclusiones:
- Este estudio presenta evidencia convincente de la presencia de peptidoglicano funcional en las especies de Chlamydia.
- Los hallazgos resuelven la "anomalía clamidial" y confirman el papel de PG en estas bacterias.
- Esto avanza en nuestra comprensión de la biología de la clamidia y los posibles objetivos terapéuticos.
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