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Los cambios en la transcripción del ARNr influyen en la proliferación y el destino celular dentro de un linaje de

Qiao Zhang¹, Nevine A Shalaby, Michael Buszczak

¹Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9148, USA.

Science (New York, N.Y.)

|January 18, 2014

Resumen

La proliferación de células madre germinales de Drosophila está regulada por un nuevo complejo de ARN polimerasa I. La modulación de la síntesis de ARN ribosómico afecta el destino celular, el crecimiento y la proliferación en estas células madre.

Área de la Ciencia:

Biología celular Biología celular.
Biología del desarrollo Biología del desarrollo.
Genética molecular La genética molecular.

Sus antecedentes:

La biogénesis del ribosoma es crucial para el crecimiento y la proliferación celular.
Los mecanismos que controlan la biogénesis de los ribosomas en tipos específicos de células no se comprenden bien.

Objetivo del estudio:

Para identificar los factores reguladores de la ARN polimerasa I (Pol I) en Drosophila.
Investigar el papel de la regulación de Pol I en la proliferación y diferenciación de las células madre de la línea germinal ovárica (GSC).

Principales métodos:

Identificación de un nuevo complejo regulatorio Pol I que involucra factores subdesarrollados (Udd), TAF1B y TAF1C.
Análisis de la proliferación y diferenciación de GSC tras la interrupción de udd o TAF1B.
Cuantificación de los niveles de transcripción del ARN ribosomal (ARNr) y la expresión de Mad en GSCs y células diferenciadoras.

Principales resultados:

La alteración de udd o TAF1B perjudica la proliferación de la GSC ovárica.
Udd está enriquecido en GSCs en comparación con las células diferenciadoras, lo que se correlaciona con una alta transcripción de ARNr.
La alteración de la transcripción de Pol I afecta la diferenciación del GSC, el destino celular y la expresión de Mad.

Conclusiones:

Un nuevo complejo regulador Pol I (Udd, TAF1B, similar a TAF1C) controla la proliferación de GSC.
La modulación de la síntesis de ARNr por este complejo influye en el destino celular, el crecimiento y la proliferación en las Drosophila GSC hembra.
Este estudio revela un vínculo entre la biogénesis del ribosoma y las vías de desarrollo como la señalización de BMP.

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