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Partículas con propiedades de retrovirus en monocitos de pacientes con cáncer de mama
A M Al-Sumidaie1, S J Leinster, C A Hart
1University Department of Surgery, University of Liverpool.
Lancet (London, England)
|January 2, 1988
Resumen
Los investigadores detectaron agentes similares a los retrovirus en los monocitos de pacientes con cáncer de mama, evidenciado por la actividad de la transcriptasa inversa y la morfología específica de las partículas. Esto sugiere un posible vínculo retroviral con el cáncer de mama, lo que justifica una mayor investigación sobre su papel.
Área de la Ciencia:
- Oncología Oncología.
- Virología Virología.
- Inmunología Inmunología.
Sus antecedentes:
- Los monocitos son células inmunes clave implicadas en varios tipos de cáncer.
- Se sabe que los retrovirus influyen en los procesos celulares y en el desarrollo de enfermedades.
Objetivo del estudio:
- Para investigar la presencia de agentes retrovirales en los monocitos de pacientes con cáncer de mama.
- Para caracterizar la naturaleza de estos agentes y su asociación con el cáncer de mama.
Principales métodos:
- Análisis de cultivo de monocitos y medio de cultivo libre de células (CFCM) para la actividad de la transcriptasa inversa (RT).
- La centrifugación de densidad flotante y el tratamiento con detergente no iónico para caracterizar las partículas.
- Microscopía electrónica de manchas negativas para visualizar la morfología viral.
Principales resultados:
- Alta prevalencia (97%) de la actividad de RT en CFCM en pacientes con cáncer de mama frente al 11% en los controles.
- Actividad de RT asociada con partículas parecidas a retrovirus (densidad 1.165-1.18 g/ml) sensibles al detergente.
- La microscopía electrónica reveló partículas envueltas que se asemejan al virus del tumor mamario murino en CFCM y macrófagos de pacientes.
Conclusiones:
- Una fuerte evidencia sugiere la presencia de un retrovirus dentro de los monocitos de pacientes con cáncer de mama.
- El papel de este retrovirus en la fisiopatología del cáncer de mama requiere una mayor aclaración.
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