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Hacia la detección nanomolar por RMN a través de la hiperpolarización SABRE

Nan Eshuis¹, Niels Hermkens, Bram J A van Weerdenburg

¹Radboud University Nijmegen , Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

Journal of the American Chemical Society

|January 31, 2014

Resumen

La hiperpolarización de espín nuclear utilizando SABRE ahora puede detectar bajas concentraciones. La adición de un ligando coordinador restaura la mejora de la señal para el análisis de RMN sensible por debajo de 1 μM.

Área de la Ciencia:

Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN) Espectroscopia de Resonancia Magnética Nuclear (RMN)
Química cuántica es la química cuántica.
Química Física es la química física.

Sus antecedentes:

SABRE (Spin-transfer por intercambio reversible iniciado por auxiliares) es una técnica de hiperpolarización.
Mejora las señales de RMN al transferir el orden de espín del para-hidrógeno (p-H2) a las moléculas del sustrato a través de un complejo metálico.
Las limitaciones actuales incluyen una mejora reducida de la señal a bajas concentraciones de sustrato (μM).

Objetivo del estudio:

Para superar las limitaciones de concentración de SABRE.
Para permitir la detección por RMN de analitos de baja concentración utilizando SABRE.
Para mejorar la eficiencia de SABRE a niveles de sustrato μM.

Principales métodos:

Investigó el efecto de la adición de ligandos coordinadores a las soluciones SABRE.
Se utilizó la formación de complejos reversibles de sustrato-metal para la transferencia de espín.
Empleado para-hidrógeno (p-H2) como la fuente de hiperpolarización.

Principales resultados:

Se demostró que la adición de un ligando coordinador adecuado restaura la eficiencia de SABRE en bajas concentraciones de sustrato.
Se logró la detección por RMN de sustratos en concentraciones inferiores a 1 μM.
Habilitado el análisis sensible de RMN en un solo escaneo.

Conclusiones:

La adición de ligandos coordinadores es una estrategia efectiva para mejorar el rendimiento de SABRE en bajas concentraciones de sustrato.
Este método amplía significativamente la aplicabilidad de SABRE para el análisis de soluciones diluidas.
La técnica ofrece una vía para la detección de RMN altamente sensible en varios campos científicos.

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