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La fosforilación alterada y la activación de pp60c-src durante la mitosis de los fibroblastos
I Chackalaparampil1, D Shalloway
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|March 25, 1988
Resumen
Durante la mitosis, pp60c-src se somete a una nueva fosforilación, aumentando su actividad quinasa de 4 a 7 veces. Esta modificación, independiente de Tyr 416/527, sugiere que pp60c-src regula los eventos específicos de la mitosis.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- pp60c-src es una tirosina quinasa no receptor involucrada en la señalización celular.
- La mitosis implica complejos mecanismos reguladores que controlan la división celular.
Objetivo del estudio:
- Investigar las modificaciones y la actividad de pp60c-src durante la mitosis.
- Para determinar si pp60c-src juega un papel en eventos específicos de la mitosis.
Principales métodos:
- Utilizó las células 3T3 de NIH que sobreexpresan c-src.
- Se analizaron los sitios de fosforilación pp60c-src (treonina, serina) durante la mitosis.
- Evaluación in vitro de la actividad de la quinasa y análisis de fosfopéptidos.
- Utilizó retardadores de movilidad electroforéticos para pp60c-src y pp60v-src endógenos.
Principales resultados:
- Más de la mitad de pp60c-src se modifica por fosforilación de threonina y serina en su región amino-terminal durante la mitosis.
- El pp60c-src mitótico exhibe de 4 a 7 veces mayor actividad de la quinasa in vitro.
- Las modificaciones son independientes de la fosforilación Tyr 416/Tyr 527.
- Las pp60c-src y pp60v-src endógenas muestran modificaciones similares durante la mitosis.
- La actividad aumentada y las modificaciones se resuelven cerca de la división celular.
Conclusiones:
- pp60c-src está sujeto a una regulación específica de la mitosis.
- La mejora de la actividad de la quinasa pp60c-src durante la mitosis sugiere un papel en la regulación de los eventos de división celular.
- Estos hallazgos destacan un vínculo potencial entre la señalización pp60c-src y la progresión mitótica.
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