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Videos de Conceptos Relacionados

Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...

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Video Experimental Relacionado

Updated: Jul 9, 2026

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
08:51

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Published on: May 8, 2012

Tenascin interfiere con la acción de la fibronectina.

R Chiquet-Ehrismann1, P Kalla, C A Pearson

  • 1Friedrich Miescher Institut, Basel, Switzerland.

Cell
|May 6, 1988
PubMed
Resumen

La tenascina inhibe la propagación celular y la fijación a sustratos como la fibronectina. El bloqueo de la tenascina.

Área de la Ciencia:

  • Biología celular Biología celular.
  • La bioquímica es la bioquímica.
  • La investigación de la matriz extracelular en la investigación de la matriz extracelular.

Sus antecedentes:

  • La fijación y la propagación celular son cruciales para el desarrollo y la función de los tejidos.
  • La matriz extracelular (ECM) juega un papel vital en la regulación del comportamiento celular.
  • La tenascina y la fibronectina son proteínas claves de la ECM con distintas interacciones celulares.

Objetivo del estudio:

  • Investigar el papel de la tenascina en la regulación de la fijación y la morfología de los fibroblastos.
  • Para determinar cómo la tenascina influye en las interacciones celulares con otros componentes del ECM.
  • Para identificar la región específica de la tenascina responsable de sus efectos inhibidores en la fijación celular.

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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Principales métodos:

  • Sustratos de recubrimiento con diferentes proporciones de tenascina y fibronectina.
  • Observando la fijación y la morfología de los fibroblastos primarios en pollitos y ratas.
  • Evaluación de los efectos de la tenascina en la adhesión celular mediada por integrina.
  • Utilizando anticuerpos monoclonales y fragmentos de Fab contra la tenascina para bloquear su actividad.
  • Empleando microscopía electrónica para localizar los sitios de unión de anticuerpos en la tenascina.

Principales resultados:

  • Los fibroblastos de pollo se adhirieron a la tenascina pero se mantuvieron redondeados, lo que indica una propagación inhibida.
  • La tenascina inhibió la unión mediada por integrina a la fibronectina, la laminina y el péptido GRGDS.
  • La tenascina también inhibió la unión de los fibroblastos de rata a la fibronectina, pero no a la laminina.
  • Los anticuerpos monoclonales y los fragmentos de Fab contra la tenascina neutralizaron sus efectos inhibidores.
  • El epítopo de estos anticuerpos se localizó en el botón terminal de los brazos de tenascin.

Conclusiones:

  • La tenascina actúa como un inhibidor de la propagación y la fijación celular, particularmente en conjunción con otras proteínas ECM.
  • La función inhibidora de la tenascina está mediada por el enmascaramiento de sitios específicos de unión celular.
  • La región del botón terminal de la tenascina contiene un epítopo crítico involucrado en la regulación de la adhesión celular.