Video Experimental Relacionado
Updated: May 2, 2026
08:55
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
Published on: August 7, 2018
10.1K
¿Se requiere SIRT2 para la necroptosis?
Kim Newton1, Joanne M Hildebrand2, Zhirong Shen3
1Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|February 28, 2014
Resumen
Este estudio investigó el papel de SIRT2 en la necroptosis, una forma de muerte celular programada. Los investigadores descubrieron que la inhibición o eliminación de SIRT2 no protegía a las células de la necroptosis, lo que cuestiona su importancia en esta vía.
Área de la Ciencia:
- Biología celular Biología celular.
- Los mecanismos moleculares de la muerte celular.
Sus antecedentes:
- Las sirtuinas, una clase de proteínas, regulan los procesos celulares a través de la desacetilación.
- Investigaciones anteriores sugirieron que SIRT2 es esencial para la necroptosis, una vía regulada de muerte celular.
Objetivo del estudio:
- Confirmar e investigar el papel de la SIRT2 en la necroptosis.
- Desafiar la hipótesis existente sobre el requisito de SIRT2 para la necroptosis.
Principales métodos:
- Utilizó múltiples inhibidores de SIRT2 (AGK2) y técnicas de silenciamiento de genes (siRNAs).
- Se emplearon modelos de ratones knockout (Sirt2-/-) y controles de tipo salvaje.
- Se evaluó la muerte celular en respuesta a la inducción del factor de necrosis tumoral (TNF) y el síndrome de respuesta inflamatoria sistémica (SIRS).
Principales resultados:
- La inhibición o el agotamiento de la SIRT2 no confiere protección contra la necroptosis.
- Los ratones Sirt2-/- exhibieron una mayor susceptibilidad al SIRS inducido por el TNF en comparación con los ratones de tipo salvaje.
- Los ratones con deficiencia de Ripk3 (Ripk3-/-) fueron resistentes a la necroptosis, sirviendo como un control positivo.
Conclusiones:
- Los hallazgos desafían el papel establecido de SIRT2 en la vía de la necroptosis.
- SIRT2 puede no ser un regulador crítico de la necroptosis como se sugirió anteriormente.
- Se necesitan más investigaciones para aclarar completamente la función de SIRT2 en la muerte celular.
Videos de Conceptos Relacionados
01:16Necrosis
5.2K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
5.2K
01:30Overview of Cell Death
7.7K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.7K
01:17The Extrinsic Apoptotic Pathway
6.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
01:18Autophagic Cell Death
3.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.3K
01:30Apoptosis
12.0K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.0K
01:31The Intrinsic Apoptotic Pathway
6.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K