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La constricción apical impulsa el flujo hidrodinámico a escala de tejido para mediar el alargamiento celular
Bing He1, Konstantin Doubrovinski1, Oleg Polyakov2
11] Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA [2].
Nature
|March 5, 2014
Resumen
La deformación del tejido durante el desarrollo depende de cómo se transmiten las fuerzas dentro de las células. Este estudio muestra que el flujo viscoso citoplasmático, no los cambios de forma de las células individuales, impulsa el plegamiento de los tejidos en los embriones de Drosophila.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- La biofísica es la biofísica.
Sus antecedentes:
- El plegamiento epitelial es crucial para la formación de tejidos durante el desarrollo animal.
- Los mecanismos que transmiten las fuerzas de la constricción apical a la deformación global del tejido no se comprenden completamente.
Objetivo del estudio:
- Investigar cómo las fuerzas generadas por la constricción apical se transmiten dentro de las células durante la morfogénesis tisular.
- Aclarar el papel del flujo citoplasmático y la individualización celular en la formación del surco ventral.
Principales métodos:
- La velocimetría de seguimiento de partículas se utilizó para medir el movimiento de las membranas citoplasmáticas y plasmáticas.
- Se realizaron experimentos en embriones de Drosophila gastrulantes, incluidos los mutantes acelulares.
Principales resultados:
- La redistribución citoplasmática durante el alargamiento del surco ventral sigue patrones de flujo viscoso predichos por la hidrodinámica.
- Las membranas celulares exhiben una resistencia o fuerza motriz mínima en el flujo citoplasmático.
- La constricción apical en embriones acelulares genera patrones de flujo citoplasmático similares a los de los embriones de tipo salvaje.
Conclusiones:
- El comportamiento hidrodinámico del citoplasma es el mecanismo principal para la transmisión de la fuerza durante el alargamiento del surco ventral.
- La individualización celular no es esencial para la deformación global del tejido observada durante este proceso.
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