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Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Una tirosina fosfatasa bacteriana inhibe la activación del receptor de reconocimiento de patrones de las plantas

Alberto P Macho¹, Benjamin Schwessinger, Vardis Ntoukakis

¹The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.

Science (New York, N.Y.)

|March 15, 2014

Resumen

Los receptores inmunes de las plantas como la quinasa del receptor de Arabidopsis EF-TU RECEPTOR (EFR) son activados por la fosforilación de la tirosina. Una fosfatasa bacteriana inhibe esto, destacando una batalla molecular para controlar la inmunidad de las plantas.

Área de la Ciencia:

Inmunidad vegetal La inmunidad vegetal.
La señalización molecular.
La patogénesis bacteriana es la patogénesis bacteriana.

Sus antecedentes:

La inmunidad innata involucra receptores de reconocimiento de patrones (PRR) que detectan patrones moleculares asociados a patógenos (PAMP).
Muchos PRR de plantas son receptoras quinasas, cruciales para reconocer a los invasores microbianos.

Objetivo del estudio:

Para investigar el mecanismo de activación del receptor EF-TU de Arabidopsis (EFR) tras la unión de ligando.
Para aclarar el papel de la fosforilación de la tirosina en la activación de la EFR y las respuestas inmunes de las plantas.

Principales métodos:

Pruebas de unión de ligandos para estudiar la activación de EFR.
Mutagénesis dirigida al sitio para identificar sitios clave de fosforilación.
Análisis de las interacciones entre plantas y patógenos utilizando Pseudomonas syringae.

Principales resultados:

EFR se activa por fosforilación en los residuos de tirosina después de unirse al péptido elf18.
La fosforilación de la tirosina 836 (Y836) es esencial para la activación de la EFR y la inmunidad a las jeringuillas de Pseudomonas.
La tirosina fosfatasa bacteriana HopAO1 desactiva la EFR reduciendo su fosforilación, suprimiendo así la inmunidad de las plantas.

Conclusiones:

La activación de la EFR y la posterior inmunidad vegetal están reguladas por la fosforilación de la tirosina.
Existe una competencia molecular entre la fosforilación de la PRR del huésped y las fosfatasas patógenas para controlar la inmunidad antibacteriana.

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