Video Experimental Relacionado
Updated: May 2, 2026
06:52
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Published on: May 21, 2018
10.2K
Inflammasoma: poner las piezas juntas
1Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universität München, 81675 Munich, Germany.
Cell
|March 18, 2014
Resumen
La activación del inflammasoma desencadena la polimerización de ASC en filamentos similares a los priones, formando plataformas para la producción de citoquinas inflamatorias. Esto revela un mecanismo unificado para el ensamblaje del inflamatorio y la liberación de citoquinas.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La activación del inflammasoma por señales microbianas y de peligro es crucial para la inmunidad innata.
- Los mecanismos precisos que subyacen a la activación del inflamatorio y la posterior liberación de citoquinas inflamatorias siguen siendo incompletamente entendidos.
Objetivo del estudio:
- Para dilucidar los mecanismos moleculares de la activación del inflamatorio.
- Identificar la base estructural para la producción inflamatoria de citoquinas después de la activación del sensor inflamatorio.
Principales métodos:
- Investigó el papel del adaptador ASC en el ensamblaje del inflamasoma.
- Utilizó técnicas para observar la polimerización de ASC en filamentos después de la activación del sensor del inflamasoma.
Principales resultados:
- Se demostró que la activación del sensor inflamatorio induce la polimerización en forma de priones de la proteína adaptadora ASC en filamentos de orden superior.
- Se demostró que estos filamentos ASC sirven como plataformas esenciales para la producción de citoquinas inflamatorias.
Conclusiones:
- Propuso un mecanismo unificado para el ensamblaje del inflamasoma, destacando el papel crítico de la polimerización tipo prión de ASC.
- Estos hallazgos proporcionan nuevos conocimientos sobre la regulación de las respuestas inflamatorias y la producción de citoquinas.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:17The Extrinsic Apoptotic Pathway
6.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
01:17Intracellular Signaling Affects Focal Adhesions
2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.8K
01:15Activation of Integrins
4.3K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.3K
01:31The Intrinsic Apoptotic Pathway
6.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
02:41Protein Complex Assembly
12.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
12.5K
01:17Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K