Video Experimental Relacionado
Updated: May 6, 2026
10:43
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
Published on: May 19, 2016
6.2K
Fosforilatos purificados del factor promotor de la maduración pp60c-src en los sitios fosforilados durante la mitosis
S Shenoy1, J K Choi, S Bagrodia
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|June 2, 1989
Resumen
El pollo pp60c-src sufre nuevas fosforilaciones mitóticas en sitios específicos por el factor promotor de la maduración (MPF). Esta fosforilación alterada explica el origen de pp60c-src.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El pp60c-src de pollo sobreexpresado exhibe movilidad y fosforilación alteradas durante la mitosis celular NIH 3T3.
- La fosforilación mitótica es crucial para regular los procesos celulares y la función de las proteínas.
Objetivo del estudio:
- Para identificar nuevos sitios de fosforilación mitótica en pp60c-src.
- Investigar el papel del factor promotor de la maduración (MPF) en la fosforilación de pp60c-src durante la mitosis.
- Para determinar si la fosforilación alterada explica los cambios observados en la movilidad pp60c-src.
Principales métodos:
- Análisis de mutagenesis y fosforilación dirigidos al sitio de pp60c-src.
- Ensayos de fosforilación in vitro utilizando MPF purificado de huevos de Xenopus.
- Pruebas de desplazamiento de movilidad electroforética para evaluar el impacto de la fosforilación.
Principales resultados:
- Se identificaron nuevas fosforilaciones mitóticas en Thr 34, Thr 46 y Ser 72 en pp60c-src.
- Estos sitios son reconocidos por MPF, la subunidad catalítica de p34cdc2.2.
- La fosforilación mediada por MPF de pp60c-src en estos sitios es suficiente para causar el retraso observado en la movilidad electroforética.
- La desfosforilación del pp60c-src mitótico restauró su movilidad normal.
Conclusiones:
- pp60c-src es un objetivo directo de la MPF durante la mitosis.
- La fosforilación de pp60c-src inducida por MPF contribuye a los cambios celulares observados durante la mitosis.
- Estos hallazgos proporcionan información sobre la regulación de la actividad de pp60c-src y su papel en la progresión mitótica.
Videos de Conceptos Relacionados
02:39Positive Regulator Molecules
5.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.3K
02:15M-Cdk Drives Transition Into Mitosis
5.4K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.4K
02:38Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
00:50Anaphase Promoting Complex
2.5K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.5K
01:07MAPK Signaling Cascades
7.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.4K
01:22PI3K/mTOR/AKT Signaling Pathway
5.2K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.2K