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La fosforilación reversible de tirosina de cdc2: la desfosforilación acompaña a la activación durante la entrada en
A O Morla1, G Draetta, D Beach
1Department of Biology, University of California, La Jolla, San Diego 92093.
Cell
|July 14, 1989
Resumen
La regulación del ciclo celular implica la fosforilación de la tirosina de la cinasa cdc2. La desfosforilación de cdc2 es crucial para la activación mitótica, destacando su papel en la progresión del ciclo celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La actividad de la Cdc2 quinasa está estrictamente regulada durante el ciclo celular.
- La fosforilación de la tirosina juega un papel en la modulación de la actividad cdc2.
Objetivo del estudio:
- Investigar el papel de la fosforilación y desfosforilación de la tirosina de cdc2 en el ciclo celular de los fibroblastos 3T3 del ratón.
- Para determinar la relación entre el estado de fosforilación de cdc2 y su actividad quinasa.
Principales métodos:
- Análisis de los niveles de fosforilación de la tirosina cdc2 en diferentes fases del ciclo celular.
- Análisis de la actividad de la histona H1 quinasa de cdc2.2.
- La inhibición de la desfosforilación de la tirosina mediante el uso de inhibidores de la fosfatasa.
Principales resultados:
- La fosforilación por tirosina de cdc2 aumenta durante las fases G1 a G2 y disminuye durante la mitosis.
- El cdc2 fosforilado por tirosina es inactivo como una histona H1 quinasa.
- La inhibición de la desfosforilación de la tirosina conduce a la detención del ciclo celular G2.
Conclusiones:
- La desfosforilación de tirosina de cdc2 es un paso esencial para su activación durante la mitosis.
- La regulación de Cdc2 implica cambios dinámicos en el estado de fosforilación de la tirosina a lo largo del ciclo celular.
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